Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21120 and BPHYT_RS21125 are separated by 173 nucleotides BPHYT_RS21125 and BPHYT_RS21130 overlap by 4 nucleotides BPHYT_RS21130 and BPHYT_RS21135 overlap by 4 nucleotides
BPHYT_RS21115: BPHYT_RS21115 - radical SAM protein, at 298,868 to 300,139
_RS21115
BPHYT_RS21120: BPHYT_RS21120 - hypothetical protein, at 300,132 to 300,959
_RS21120
BPHYT_RS21125: BPHYT_RS21125 - hypothetical protein, at 301,133 to 301,774
_RS21125
BPHYT_RS21130: BPHYT_RS21130 - F420-dependent oxidoreductase, at 301,771 to 302,550
_RS21130
BPHYT_RS21135: BPHYT_RS21135 - NADH-ubiquinone oxidoreductase subunit 6, at 302,547 to 303,227
_RS21135
Position (kb) 301
302 Strain fitness (log2 ratio) -2
-1
0
1
2 at 300.313 kb on - strand, within BPHYT_RS21120 at 300.434 kb on + strand, within BPHYT_RS21120 at 300.434 kb on + strand, within BPHYT_RS21120 at 300.435 kb on - strand, within BPHYT_RS21120 at 300.507 kb on - strand, within BPHYT_RS21120 at 300.535 kb on + strand, within BPHYT_RS21120 at 300.535 kb on + strand, within BPHYT_RS21120 at 300.536 kb on - strand, within BPHYT_RS21120 at 300.918 kb on + strand at 301.132 kb on + strand at 301.809 kb on - strand at 301.969 kb on + strand, within BPHYT_RS21130 at 301.969 kb on + strand, within BPHYT_RS21130 at 301.969 kb on + strand, within BPHYT_RS21130 at 302.621 kb on - strand, within BPHYT_RS21135 at 302.692 kb on + strand, within BPHYT_RS21135 at 302.693 kb on - strand, within BPHYT_RS21135 at 302.698 kb on + strand, within BPHYT_RS21135 at 302.698 kb on + strand, within BPHYT_RS21135
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 300,313 - BPHYT_RS21120 0.22 +1.3 300,434 + BPHYT_RS21120 0.36 -0.1 300,434 + BPHYT_RS21120 0.36 -0.8 300,435 - BPHYT_RS21120 0.37 -1.9 300,507 - BPHYT_RS21120 0.45 +0.1 300,535 + BPHYT_RS21120 0.49 -0.2 300,535 + BPHYT_RS21120 0.49 -0.7 300,536 - BPHYT_RS21120 0.49 -0.3 300,918 + -0.8 301,132 + +0.8 301,809 - +0.1 301,969 + BPHYT_RS21130 0.25 -0.6 301,969 + BPHYT_RS21130 0.25 +2.1 301,969 + BPHYT_RS21130 0.25 -2.3 302,621 - BPHYT_RS21135 0.11 -0.0 302,692 + BPHYT_RS21135 0.21 -0.9 302,693 - BPHYT_RS21135 0.21 -2.3 302,698 + BPHYT_RS21135 0.22 -0.4 302,698 + BPHYT_RS21135 0.22 -2.1
Or see this region's nucleotide sequence