Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20915 and BPHYT_RS20920 are separated by 11 nucleotides BPHYT_RS20920 and BPHYT_RS20925 are separated by 400 nucleotides BPHYT_RS20925 and BPHYT_RS20930 are separated by 584 nucleotides BPHYT_RS20930 and BPHYT_RS20935 are separated by 37 nucleotides
BPHYT_RS20915: BPHYT_RS20915 - hypothetical protein, at 254,427 to 255,434
_RS20915
BPHYT_RS20920: BPHYT_RS20920 - oxidoreductase, at 255,446 to 255,991
_RS20920
BPHYT_RS20925: BPHYT_RS20925 - dimethylallyltransferase, at 256,392 to 257,069
_RS20925
BPHYT_RS20930: BPHYT_RS20930 - hypothetical protein, at 257,654 to 257,941
_RS20930
BPHYT_RS20935: BPHYT_RS20935 - dehydrogenase, at 257,979 to 258,413
_RS20935
Position (kb) 256
257
258 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 255.607 kb on - strand, within BPHYT_RS20920 at 255.607 kb on - strand, within BPHYT_RS20920 at 255.618 kb on + strand, within BPHYT_RS20920 at 255.836 kb on + strand, within BPHYT_RS20920 at 255.837 kb on - strand, within BPHYT_RS20920 at 255.837 kb on - strand, within BPHYT_RS20920 at 256.216 kb on + strand at 256.304 kb on + strand at 256.775 kb on - strand, within BPHYT_RS20925 at 256.775 kb on - strand, within BPHYT_RS20925 at 256.775 kb on - strand, within BPHYT_RS20925 at 256.976 kb on - strand, within BPHYT_RS20925 at 257.431 kb on + strand at 257.432 kb on - strand at 257.541 kb on + strand at 257.541 kb on + strand at 257.558 kb on + strand at 257.596 kb on + strand at 257.596 kb on + strand at 257.596 kb on + strand at 257.599 kb on + strand at 257.648 kb on + strand at 257.649 kb on - strand at 257.649 kb on - strand at 257.649 kb on - strand at 257.664 kb on - strand at 257.715 kb on - strand, within BPHYT_RS20930 at 257.715 kb on - strand, within BPHYT_RS20930 at 257.981 kb on - strand at 257.988 kb on - strand at 257.988 kb on - strand at 258.037 kb on - strand, within BPHYT_RS20935 at 258.047 kb on + strand, within BPHYT_RS20935 at 258.048 kb on - strand, within BPHYT_RS20935
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 255,607 - BPHYT_RS20920 0.29 -0.5 255,607 - BPHYT_RS20920 0.29 +0.1 255,618 + BPHYT_RS20920 0.32 -1.2 255,836 + BPHYT_RS20920 0.71 -0.2 255,837 - BPHYT_RS20920 0.72 -0.6 255,837 - BPHYT_RS20920 0.72 +0.8 256,216 + +0.2 256,304 + -0.3 256,775 - BPHYT_RS20925 0.56 -1.1 256,775 - BPHYT_RS20925 0.56 -1.4 256,775 - BPHYT_RS20925 0.56 -0.7 256,976 - BPHYT_RS20925 0.86 -1.0 257,431 + -1.8 257,432 - +0.2 257,541 + -0.5 257,541 + +1.0 257,558 + -1.1 257,596 + +1.3 257,596 + +0.3 257,596 + +0.8 257,599 + -1.1 257,648 + -0.4 257,649 - +1.2 257,649 - -2.8 257,649 - -1.2 257,664 - +0.2 257,715 - BPHYT_RS20930 0.21 -0.5 257,715 - BPHYT_RS20930 0.21 -1.2 257,981 - +1.4 257,988 - -0.7 257,988 - -1.3 258,037 - BPHYT_RS20935 0.13 -0.5 258,047 + BPHYT_RS20935 0.16 +1.1 258,048 - BPHYT_RS20935 0.16 +0.8
Or see this region's nucleotide sequence