Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20680

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20675 and BPHYT_RS20680 are separated by 191 nucleotidesBPHYT_RS20680 and BPHYT_RS20685 are separated by 72 nucleotides BPHYT_RS20675: BPHYT_RS20675 - LysR family transcriptional regulator, at 198,610 to 199,482 _RS20675 BPHYT_RS20680: BPHYT_RS20680 - LacI family transcriptional regulator, at 199,674 to 200,639 _RS20680 BPHYT_RS20685: BPHYT_RS20685 - ribose ABC transporter ATPase, at 200,712 to 202,325 _RS20685 Position (kb) 199 200 201Strain fitness (log2 ratio) -2 -1 0 1at 198.962 kb on + strand, within BPHYT_RS20675at 198.962 kb on + strand, within BPHYT_RS20675at 199.016 kb on + strand, within BPHYT_RS20675at 199.016 kb on + strand, within BPHYT_RS20675at 199.082 kb on + strand, within BPHYT_RS20675at 199.163 kb on + strand, within BPHYT_RS20675at 199.241 kb on - strand, within BPHYT_RS20675at 199.537 kb on - strandat 200.195 kb on + strand, within BPHYT_RS20680at 200.521 kb on - strand, within BPHYT_RS20680at 200.521 kb on - strand, within BPHYT_RS20680at 200.781 kb on + strandat 201.330 kb on + strand, within BPHYT_RS20685at 201.331 kb on - strand, within BPHYT_RS20685at 201.457 kb on + strand, within BPHYT_RS20685at 201.501 kb on - strand, within BPHYT_RS20685

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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198,962 + BPHYT_RS20675 0.40 -0.1
198,962 + BPHYT_RS20675 0.40 +0.1
199,016 + BPHYT_RS20675 0.47 -0.7
199,016 + BPHYT_RS20675 0.47 -0.7
199,082 + BPHYT_RS20675 0.54 -1.1
199,163 + BPHYT_RS20675 0.63 +0.7
199,241 - BPHYT_RS20675 0.72 +0.5
199,537 - -2.3
200,195 + BPHYT_RS20680 0.54 -0.8
200,521 - BPHYT_RS20680 0.88 -0.2
200,521 - BPHYT_RS20680 0.88 -1.5
200,781 + -0.3
201,330 + BPHYT_RS20685 0.38 +0.4
201,331 - BPHYT_RS20685 0.38 -0.3
201,457 + BPHYT_RS20685 0.46 +0.8
201,501 - BPHYT_RS20685 0.49 +0.2

Or see this region's nucleotide sequence