Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20545

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20535 and BPHYT_RS20540 overlap by 19 nucleotidesBPHYT_RS20540 and BPHYT_RS20545 are separated by 87 nucleotidesBPHYT_RS20545 and BPHYT_RS20550 are separated by 160 nucleotides BPHYT_RS20535: BPHYT_RS20535 - allantoate amidohydrolase, at 169,451 to 170,692 _RS20535 BPHYT_RS20540: BPHYT_RS20540 - N-acetyltransferase GCN5, at 170,674 to 171,555 _RS20540 BPHYT_RS20545: BPHYT_RS20545 - hypothetical protein, at 171,643 to 171,948 _RS20545 BPHYT_RS20550: BPHYT_RS20550 - methylated-DNA-protein-cysteine methyltransferase, at 172,109 to 172,741 _RS20550 Position (kb) 171 172Strain fitness (log2 ratio) -2 -1 0 1 2at 170.808 kb on + strand, within BPHYT_RS20540at 170.911 kb on + strand, within BPHYT_RS20540at 170.912 kb on - strand, within BPHYT_RS20540at 170.912 kb on - strand, within BPHYT_RS20540at 170.912 kb on - strand, within BPHYT_RS20540at 170.914 kb on - strand, within BPHYT_RS20540at 170.977 kb on + strand, within BPHYT_RS20540at 171.031 kb on + strand, within BPHYT_RS20540at 171.032 kb on - strand, within BPHYT_RS20540at 171.112 kb on + strand, within BPHYT_RS20540at 171.488 kb on - strandat 171.509 kb on + strandat 171.644 kb on + strandat 171.645 kb on - strandat 171.831 kb on - strand, within BPHYT_RS20545at 171.859 kb on + strand, within BPHYT_RS20545at 171.929 kb on - strandat 171.981 kb on + strandat 171.987 kb on + strandat 171.988 kb on - strandat 172.018 kb on - strandat 172.734 kb on + strandat 172.735 kb on - strandat 172.735 kb on - strandat 172.797 kb on + strandat 172.934 kb on + strandat 172.934 kb on + strandat 172.934 kb on + strandat 172.935 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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170,808 + BPHYT_RS20540 0.15 -0.1
170,911 + BPHYT_RS20540 0.27 -0.5
170,912 - BPHYT_RS20540 0.27 -0.8
170,912 - BPHYT_RS20540 0.27 +0.2
170,912 - BPHYT_RS20540 0.27 -0.0
170,914 - BPHYT_RS20540 0.27 -0.1
170,977 + BPHYT_RS20540 0.34 +0.5
171,031 + BPHYT_RS20540 0.40 +0.6
171,032 - BPHYT_RS20540 0.41 -1.0
171,112 + BPHYT_RS20540 0.50 +0.7
171,488 - -1.2
171,509 + -0.7
171,644 + -1.3
171,645 - -1.0
171,831 - BPHYT_RS20545 0.61 -1.8
171,859 + BPHYT_RS20545 0.71 +0.1
171,929 - -0.4
171,981 + +0.3
171,987 + +0.6
171,988 - -0.3
172,018 - -0.7
172,734 + -1.2
172,735 - -0.0
172,735 - +0.5
172,797 + -1.9
172,934 + -0.8
172,934 + +1.0
172,934 + +2.1
172,935 - -0.6

Or see this region's nucleotide sequence