Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20535 and BPHYT_RS20540 overlap by 19 nucleotides BPHYT_RS20540 and BPHYT_RS20545 are separated by 87 nucleotides BPHYT_RS20545 and BPHYT_RS20550 are separated by 160 nucleotides
BPHYT_RS20535: BPHYT_RS20535 - allantoate amidohydrolase, at 169,451 to 170,692
_RS20535
BPHYT_RS20540: BPHYT_RS20540 - N-acetyltransferase GCN5, at 170,674 to 171,555
_RS20540
BPHYT_RS20545: BPHYT_RS20545 - hypothetical protein, at 171,643 to 171,948
_RS20545
BPHYT_RS20550: BPHYT_RS20550 - methylated-DNA-protein-cysteine methyltransferase, at 172,109 to 172,741
_RS20550
Position (kb)
171
172 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 170.808 kb on + strand, within BPHYT_RS20540 at 170.911 kb on + strand, within BPHYT_RS20540 at 170.912 kb on - strand, within BPHYT_RS20540 at 170.912 kb on - strand, within BPHYT_RS20540 at 170.912 kb on - strand, within BPHYT_RS20540 at 170.914 kb on - strand, within BPHYT_RS20540 at 170.977 kb on + strand, within BPHYT_RS20540 at 171.031 kb on + strand, within BPHYT_RS20540 at 171.032 kb on - strand, within BPHYT_RS20540 at 171.112 kb on + strand, within BPHYT_RS20540 at 171.488 kb on - strand at 171.509 kb on + strand at 171.644 kb on + strand at 171.645 kb on - strand at 171.831 kb on - strand, within BPHYT_RS20545 at 171.859 kb on + strand, within BPHYT_RS20545 at 171.929 kb on - strand at 171.981 kb on + strand at 171.987 kb on + strand at 171.988 kb on - strand at 172.018 kb on - strand at 172.734 kb on + strand at 172.735 kb on - strand at 172.735 kb on - strand at 172.797 kb on + strand at 172.934 kb on + strand at 172.934 kb on + strand at 172.934 kb on + strand at 172.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 170,808 + BPHYT_RS20540 0.15 -0.1 170,911 + BPHYT_RS20540 0.27 -0.5 170,912 - BPHYT_RS20540 0.27 -0.8 170,912 - BPHYT_RS20540 0.27 +0.2 170,912 - BPHYT_RS20540 0.27 -0.0 170,914 - BPHYT_RS20540 0.27 -0.1 170,977 + BPHYT_RS20540 0.34 +0.5 171,031 + BPHYT_RS20540 0.40 +0.6 171,032 - BPHYT_RS20540 0.41 -1.0 171,112 + BPHYT_RS20540 0.50 +0.7 171,488 - -1.2 171,509 + -0.7 171,644 + -1.3 171,645 - -1.0 171,831 - BPHYT_RS20545 0.61 -1.8 171,859 + BPHYT_RS20545 0.71 +0.1 171,929 - -0.4 171,981 + +0.3 171,987 + +0.6 171,988 - -0.3 172,018 - -0.7 172,734 + -1.2 172,735 - -0.0 172,735 - +0.5 172,797 + -1.9 172,934 + -0.8 172,934 + +1.0 172,934 + +2.1 172,935 - -0.6
Or see this region's nucleotide sequence