Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20165 and BPHYT_RS20170 are separated by 168 nucleotides BPHYT_RS20170 and BPHYT_RS20175 are separated by 56 nucleotides BPHYT_RS20175 and BPHYT_RS20180 overlap by 4 nucleotides
BPHYT_RS20165: BPHYT_RS20165 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 66,730 to 67,968
_RS20165
BPHYT_RS20170: BPHYT_RS20170 - sugar transporter, at 68,137 to 70,359
_RS20170
BPHYT_RS20175: BPHYT_RS20175 - protein-tyrosine-phosphatase, at 70,416 to 70,868
_RS20175
BPHYT_RS20180: BPHYT_RS20180 - sugar ABC transporter substrate-binding protein, at 70,865 to 71,917
_RS20180
Position (kb) 68
69
70
71 Strain fitness (log2 ratio) -1
0
1 at 67.514 kb on - strand, within BPHYT_RS20165 at 67.676 kb on - strand, within BPHYT_RS20165 at 67.728 kb on + strand, within BPHYT_RS20165 at 68.162 kb on + strand at 68.450 kb on - strand, within BPHYT_RS20170 at 68.727 kb on + strand, within BPHYT_RS20170 at 69.303 kb on - strand, within BPHYT_RS20170 at 69.871 kb on - strand, within BPHYT_RS20170 at 70.860 kb on - strand at 70.889 kb on + strand at 71.069 kb on + strand, within BPHYT_RS20180 at 71.182 kb on + strand, within BPHYT_RS20180 at 71.251 kb on + strand, within BPHYT_RS20180
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 67,514 - BPHYT_RS20165 0.63 -0.6 67,676 - BPHYT_RS20165 0.76 +0.1 67,728 + BPHYT_RS20165 0.81 -0.9 68,162 + +0.4 68,450 - BPHYT_RS20170 0.14 +1.5 68,727 + BPHYT_RS20170 0.27 +1.5 69,303 - BPHYT_RS20170 0.52 -0.4 69,871 - BPHYT_RS20170 0.78 -0.0 70,860 - +1.7 70,889 + +1.5 71,069 + BPHYT_RS20180 0.19 -0.3 71,182 + BPHYT_RS20180 0.30 +1.1 71,251 + BPHYT_RS20180 0.37 -1.1
Or see this region's nucleotide sequence