Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS18150

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS18145 and BPHYT_RS18150 are separated by 218 nucleotidesBPHYT_RS18150 and BPHYT_RS18155 are separated by 21 nucleotides BPHYT_RS18145: BPHYT_RS18145 - ATP-dependent DNA helicase RecQ, at 4,080,205 to 4,082,052 _RS18145 BPHYT_RS18150: BPHYT_RS18150 - DNA-directed RNA polymerase subunit beta', at 4,082,271 to 4,086,509 _RS18150 BPHYT_RS18155: BPHYT_RS18155 - DNA-directed RNA polymerase subunit beta, at 4,086,531 to 4,090,637 _RS18155 Position (kb) 4082 4083 4084 4085 4086 4087Strain fitness (log2 ratio) -1 0 1at 4081.308 kb on + strand, within BPHYT_RS18145at 4081.326 kb on - strand, within BPHYT_RS18145at 4081.584 kb on - strand, within BPHYT_RS18145at 4081.584 kb on - strand, within BPHYT_RS18145at 4081.649 kb on - strand, within BPHYT_RS18145at 4081.649 kb on - strand, within BPHYT_RS18145at 4081.651 kb on - strand, within BPHYT_RS18145at 4081.651 kb on - strand, within BPHYT_RS18145at 4081.710 kb on - strand, within BPHYT_RS18145at 4081.710 kb on - strand, within BPHYT_RS18145at 4081.787 kb on + strand, within BPHYT_RS18145at 4082.013 kb on - strandat 4082.093 kb on + strandat 4082.150 kb on + strandat 4082.150 kb on + strandat 4082.151 kb on - strandat 4082.151 kb on - strandat 4082.151 kb on - strandat 4082.170 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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4,081,308 + BPHYT_RS18145 0.60 -0.6
4,081,326 - BPHYT_RS18145 0.61 -0.1
4,081,584 - BPHYT_RS18145 0.75 +0.4
4,081,584 - BPHYT_RS18145 0.75 +1.6
4,081,649 - BPHYT_RS18145 0.78 +0.3
4,081,649 - BPHYT_RS18145 0.78 +1.6
4,081,651 - BPHYT_RS18145 0.78 -0.8
4,081,651 - BPHYT_RS18145 0.78 -1.7
4,081,710 - BPHYT_RS18145 0.81 +1.3
4,081,710 - BPHYT_RS18145 0.81 +0.2
4,081,787 + BPHYT_RS18145 0.86 -0.0
4,082,013 - -0.1
4,082,093 + +0.2
4,082,150 + +0.2
4,082,150 + -0.1
4,082,151 - -0.2
4,082,151 - -0.7
4,082,151 - -0.5
4,082,170 - -1.1

Or see this region's nucleotide sequence