Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17425 and BPHYT_RS17430 are separated by 94 nucleotides BPHYT_RS17430 and BPHYT_RS17435 are separated by 127 nucleotides BPHYT_RS17435 and BPHYT_RS17440 are separated by 102 nucleotides BPHYT_RS17440 and BPHYT_RS17445 are separated by 316 nucleotides
BPHYT_RS17425: BPHYT_RS17425 - peptidase M61, at 3,946,941 to 3,948,731
_RS17425
BPHYT_RS17430: BPHYT_RS17430 - thiol:disulfide interchange protein, at 3,948,826 to 3,949,551
_RS17430
BPHYT_RS17435: BPHYT_RS17435 - ubiquinone biosynthesis hydroxylase UbiH, at 3,949,679 to 3,950,851
_RS17435
BPHYT_RS17440: BPHYT_RS17440 - hypothetical protein, at 3,950,954 to 3,951,319
_RS17440
BPHYT_RS17445: BPHYT_RS17445 - GTP-binding protein YchF, at 3,951,636 to 3,952,730
_RS17445
Position (kb)
3949
3950
3951 Strain fitness (log2 ratio)
-2
-1
0
1 at 3948.716 kb on - strand at 3949.106 kb on + strand, within BPHYT_RS17430 at 3949.107 kb on - strand, within BPHYT_RS17430 at 3949.107 kb on - strand, within BPHYT_RS17430 at 3949.331 kb on + strand, within BPHYT_RS17430 at 3949.332 kb on - strand, within BPHYT_RS17430 at 3949.383 kb on - strand, within BPHYT_RS17430 at 3949.641 kb on + strand at 3949.642 kb on - strand at 3950.861 kb on - strand at 3950.862 kb on + strand at 3951.108 kb on + strand, within BPHYT_RS17440 at 3951.108 kb on + strand, within BPHYT_RS17440 at 3951.108 kb on + strand, within BPHYT_RS17440 at 3951.180 kb on - strand, within BPHYT_RS17440 at 3951.308 kb on + strand at 3951.309 kb on - strand at 3951.309 kb on - strand at 3951.364 kb on + strand at 3951.365 kb on - strand at 3951.386 kb on + strand at 3951.387 kb on - strand at 3951.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,948,716 - -0.2 3,949,106 + BPHYT_RS17430 0.39 -1.8 3,949,107 - BPHYT_RS17430 0.39 -0.8 3,949,107 - BPHYT_RS17430 0.39 -1.0 3,949,331 + BPHYT_RS17430 0.70 -1.8 3,949,332 - BPHYT_RS17430 0.70 -0.8 3,949,383 - BPHYT_RS17430 0.77 -0.9 3,949,641 + -0.1 3,949,642 - -0.7 3,950,861 - +1.0 3,950,862 + -1.3 3,951,108 + BPHYT_RS17440 0.42 +0.2 3,951,108 + BPHYT_RS17440 0.42 -1.3 3,951,108 + BPHYT_RS17440 0.42 -1.4 3,951,180 - BPHYT_RS17440 0.62 -0.3 3,951,308 + -2.0 3,951,309 - -2.0 3,951,309 - -0.1 3,951,364 + +0.7 3,951,365 - -0.5 3,951,386 + +1.0 3,951,387 - +1.7 3,951,614 - +1.6
Or see this region's nucleotide sequence