Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS16120

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS16115 and BPHYT_RS16120 are separated by 72 nucleotidesBPHYT_RS16120 and BPHYT_RS16125 are separated by 332 nucleotidesBPHYT_RS16125 and BPHYT_RS16130 are separated by 59 nucleotides BPHYT_RS16115: BPHYT_RS16115 - ABC transporter for D-Sorbitol, periplasmic substrate-binding component (from data), at 3,655,894 to 3,657,216 _RS16115 BPHYT_RS16120: BPHYT_RS16120 - sorbitol dehydrogenase, D-fructose forming (EC 1.1.1.14) (from data), at 3,657,289 to 3,658,071 _RS16120 BPHYT_RS16125: BPHYT_RS16125 - Fur family transcriptional regulator, at 3,658,404 to 3,658,898 _RS16125 BPHYT_RS16130: BPHYT_RS16130 - cation ABC transporter substrate-binding protein, at 3,658,958 to 3,659,926 _RS16130 Position (kb) 3657 3658 3659Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 3656.400 kb on - strand, within BPHYT_RS16115at 3656.515 kb on + strand, within BPHYT_RS16115at 3656.528 kb on + strand, within BPHYT_RS16115at 3656.529 kb on - strand, within BPHYT_RS16115at 3656.529 kb on - strand, within BPHYT_RS16115at 3656.627 kb on + strandat 3656.675 kb on + strand, within BPHYT_RS16115at 3656.730 kb on - strand, within BPHYT_RS16115at 3656.786 kb on + strand, within BPHYT_RS16115at 3656.786 kb on + strand, within BPHYT_RS16115at 3656.786 kb on + strand, within BPHYT_RS16115at 3656.787 kb on - strand, within BPHYT_RS16115at 3656.807 kb on + strand, within BPHYT_RS16115at 3656.808 kb on - strand, within BPHYT_RS16115at 3656.808 kb on - strand, within BPHYT_RS16115at 3656.822 kb on + strand, within BPHYT_RS16115at 3656.822 kb on + strand, within BPHYT_RS16115at 3657.240 kb on + strandat 3657.244 kb on + strandat 3657.244 kb on + strandat 3657.244 kb on + strandat 3657.461 kb on + strand, within BPHYT_RS16120at 3657.461 kb on + strand, within BPHYT_RS16120at 3657.501 kb on + strand, within BPHYT_RS16120at 3657.576 kb on - strand, within BPHYT_RS16120at 3657.651 kb on + strand, within BPHYT_RS16120at 3657.651 kb on + strand, within BPHYT_RS16120at 3657.652 kb on - strand, within BPHYT_RS16120at 3657.915 kb on - strand, within BPHYT_RS16120at 3657.915 kb on - strand, within BPHYT_RS16120at 3657.993 kb on - strandat 3658.253 kb on + strandat 3658.253 kb on + strandat 3658.254 kb on - strandat 3658.254 kb on - strandat 3658.254 kb on - strandat 3658.254 kb on - strandat 3658.257 kb on - strandat 3658.322 kb on - strandat 3658.507 kb on - strand, within BPHYT_RS16125at 3658.507 kb on - strand, within BPHYT_RS16125at 3658.545 kb on + strand, within BPHYT_RS16125at 3658.546 kb on - strand, within BPHYT_RS16125at 3658.600 kb on + strand, within BPHYT_RS16125at 3658.600 kb on + strand, within BPHYT_RS16125at 3658.600 kb on + strand, within BPHYT_RS16125at 3658.600 kb on + strand, within BPHYT_RS16125at 3658.600 kb on + strand, within BPHYT_RS16125at 3658.914 kb on + strandat 3658.914 kb on + strandat 3658.914 kb on + strandat 3658.914 kb on + strandat 3658.915 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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3,656,400 - BPHYT_RS16115 0.38 +2.1
3,656,515 + BPHYT_RS16115 0.47 -0.8
3,656,528 + BPHYT_RS16115 0.48 -2.1
3,656,529 - BPHYT_RS16115 0.48 -0.4
3,656,529 - BPHYT_RS16115 0.48 +0.5
3,656,627 + -0.8
3,656,675 + BPHYT_RS16115 0.59 +0.9
3,656,730 - BPHYT_RS16115 0.63 -0.2
3,656,786 + BPHYT_RS16115 0.67 -0.4
3,656,786 + BPHYT_RS16115 0.67 -1.2
3,656,786 + BPHYT_RS16115 0.67 +0.2
3,656,787 - BPHYT_RS16115 0.67 +2.5
3,656,807 + BPHYT_RS16115 0.69 -0.9
3,656,808 - BPHYT_RS16115 0.69 -0.6
3,656,808 - BPHYT_RS16115 0.69 +1.3
3,656,822 + BPHYT_RS16115 0.70 +0.0
3,656,822 + BPHYT_RS16115 0.70 +1.6
3,657,240 + +0.7
3,657,244 + +1.5
3,657,244 + +1.1
3,657,244 + -0.8
3,657,461 + BPHYT_RS16120 0.22 +0.0
3,657,461 + BPHYT_RS16120 0.22 -0.0
3,657,501 + BPHYT_RS16120 0.27 +0.7
3,657,576 - BPHYT_RS16120 0.37 -0.6
3,657,651 + BPHYT_RS16120 0.46 -0.1
3,657,651 + BPHYT_RS16120 0.46 +5.1
3,657,652 - BPHYT_RS16120 0.46 +2.5
3,657,915 - BPHYT_RS16120 0.80 -0.8
3,657,915 - BPHYT_RS16120 0.80 -0.3
3,657,993 - +0.7
3,658,253 + -0.2
3,658,253 + +1.5
3,658,254 - -1.5
3,658,254 - +0.8
3,658,254 - +0.0
3,658,254 - -0.6
3,658,257 - -3.2
3,658,322 - +0.6
3,658,507 - BPHYT_RS16125 0.21 +0.1
3,658,507 - BPHYT_RS16125 0.21 +1.3
3,658,545 + BPHYT_RS16125 0.28 -0.8
3,658,546 - BPHYT_RS16125 0.29 +0.5
3,658,600 + BPHYT_RS16125 0.40 -0.5
3,658,600 + BPHYT_RS16125 0.40 -0.0
3,658,600 + BPHYT_RS16125 0.40 -1.6
3,658,600 + BPHYT_RS16125 0.40 -1.2
3,658,600 + BPHYT_RS16125 0.40 +0.2
3,658,914 + -0.1
3,658,914 + -1.5
3,658,914 + -0.2
3,658,914 + -0.8
3,658,915 - -0.9

Or see this region's nucleotide sequence