Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15605 and BPHYT_RS15610 are separated by 230 nucleotides BPHYT_RS15610 and BPHYT_RS15615 are separated by 540 nucleotides
BPHYT_RS15605: BPHYT_RS15605 - ABC transporter permease, at 3,539,596 to 3,540,546
_RS15605
BPHYT_RS15610: BPHYT_RS15610 - branched-chain amino acid ABC transporter substrate-binding protein, at 3,540,777 to 3,541,976
_RS15610
BPHYT_RS15615: BPHYT_RS15615 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 3,542,517 to 3,543,476
_RS15615
Position (kb)
3540
3541
3542 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3539.849 kb on + strand, within BPHYT_RS15605 at 3539.849 kb on + strand, within BPHYT_RS15605 at 3539.849 kb on + strand, within BPHYT_RS15605 at 3539.849 kb on + strand, within BPHYT_RS15605 at 3539.849 kb on + strand, within BPHYT_RS15605 at 3539.849 kb on + strand, within BPHYT_RS15605 at 3539.849 kb on + strand, within BPHYT_RS15605 at 3539.849 kb on + strand, within BPHYT_RS15605 at 3539.850 kb on - strand, within BPHYT_RS15605 at 3539.850 kb on - strand, within BPHYT_RS15605 at 3539.850 kb on - strand, within BPHYT_RS15605 at 3539.867 kb on + strand, within BPHYT_RS15605 at 3540.290 kb on + strand, within BPHYT_RS15605 at 3540.291 kb on - strand, within BPHYT_RS15605 at 3540.385 kb on + strand, within BPHYT_RS15605 at 3540.471 kb on + strand at 3540.471 kb on + strand at 3540.472 kb on - strand at 3540.472 kb on - strand at 3540.472 kb on - strand at 3540.495 kb on - strand at 3540.540 kb on + strand at 3540.541 kb on - strand at 3540.548 kb on + strand at 3540.548 kb on + strand at 3540.549 kb on - strand at 3540.549 kb on - strand at 3540.549 kb on - strand at 3540.549 kb on - strand at 3540.549 kb on - strand at 3540.549 kb on - strand at 3540.558 kb on + strand at 3540.646 kb on - strand at 3540.827 kb on - strand at 3540.896 kb on - strand at 3540.896 kb on - strand at 3540.896 kb on - strand at 3540.973 kb on + strand, within BPHYT_RS15610 at 3540.974 kb on - strand, within BPHYT_RS15610 at 3541.033 kb on + strand, within BPHYT_RS15610 at 3541.034 kb on - strand, within BPHYT_RS15610 at 3541.272 kb on - strand at 3541.344 kb on + strand, within BPHYT_RS15610 at 3541.345 kb on - strand, within BPHYT_RS15610 at 3541.345 kb on - strand, within BPHYT_RS15610 at 3541.438 kb on + strand, within BPHYT_RS15610 at 3541.439 kb on - strand, within BPHYT_RS15610 at 3541.439 kb on - strand, within BPHYT_RS15610 at 3541.487 kb on - strand, within BPHYT_RS15610 at 3541.513 kb on + strand, within BPHYT_RS15610 at 3541.562 kb on + strand, within BPHYT_RS15610 at 3541.562 kb on + strand, within BPHYT_RS15610 at 3541.562 kb on + strand, within BPHYT_RS15610 at 3541.562 kb on + strand, within BPHYT_RS15610 at 3541.562 kb on + strand, within BPHYT_RS15610 at 3541.562 kb on + strand, within BPHYT_RS15610 at 3541.562 kb on + strand, within BPHYT_RS15610 at 3541.562 kb on + strand, within BPHYT_RS15610 at 3541.563 kb on - strand, within BPHYT_RS15610 at 3541.563 kb on - strand, within BPHYT_RS15610 at 3541.563 kb on - strand, within BPHYT_RS15610 at 3541.564 kb on + strand, within BPHYT_RS15610 at 3541.564 kb on + strand, within BPHYT_RS15610 at 3541.564 kb on + strand, within BPHYT_RS15610 at 3541.564 kb on + strand, within BPHYT_RS15610 at 3541.565 kb on - strand, within BPHYT_RS15610 at 3541.565 kb on - strand, within BPHYT_RS15610 at 3541.565 kb on - strand, within BPHYT_RS15610 at 3541.565 kb on - strand, within BPHYT_RS15610 at 3541.565 kb on - strand, within BPHYT_RS15610 at 3541.565 kb on - strand, within BPHYT_RS15610 at 3541.565 kb on - strand, within BPHYT_RS15610 at 3541.566 kb on + strand, within BPHYT_RS15610 at 3541.566 kb on + strand, within BPHYT_RS15610 at 3541.566 kb on + strand, within BPHYT_RS15610 at 3541.567 kb on - strand, within BPHYT_RS15610 at 3541.567 kb on - strand at 3541.567 kb on - strand, within BPHYT_RS15610 at 3541.977 kb on + strand at 3541.978 kb on - strand at 3541.978 kb on - strand at 3541.981 kb on + strand at 3541.982 kb on - strand at 3542.007 kb on - strand at 3542.022 kb on - strand at 3542.070 kb on + strand at 3542.070 kb on + strand at 3542.070 kb on + strand at 3542.071 kb on - strand at 3542.251 kb on + strand at 3542.252 kb on - strand at 3542.255 kb on - strand at 3542.291 kb on - strand at 3542.291 kb on - strand at 3542.319 kb on + strand at 3542.350 kb on + strand at 3542.350 kb on + strand at 3542.350 kb on + strand at 3542.350 kb on + strand at 3542.350 kb on + strand at 3542.356 kb on + strand at 3542.356 kb on + strand at 3542.409 kb on + strand at 3542.409 kb on + strand at 3542.410 kb on - strand at 3542.728 kb on + strand, within BPHYT_RS15615 at 3542.864 kb on - strand, within BPHYT_RS15615
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,539,849 + BPHYT_RS15605 0.27 -1.7 3,539,849 + BPHYT_RS15605 0.27 +0.1 3,539,849 + BPHYT_RS15605 0.27 -1.3 3,539,849 + BPHYT_RS15605 0.27 +0.0 3,539,849 + BPHYT_RS15605 0.27 -0.8 3,539,849 + BPHYT_RS15605 0.27 +1.0 3,539,849 + BPHYT_RS15605 0.27 -1.6 3,539,849 + BPHYT_RS15605 0.27 +1.1 3,539,850 - BPHYT_RS15605 0.27 -0.9 3,539,850 - BPHYT_RS15605 0.27 -1.1 3,539,850 - BPHYT_RS15605 0.27 -0.9 3,539,867 + BPHYT_RS15605 0.28 -0.7 3,540,290 + BPHYT_RS15605 0.73 +0.8 3,540,291 - BPHYT_RS15605 0.73 +0.8 3,540,385 + BPHYT_RS15605 0.83 -2.0 3,540,471 + +0.7 3,540,471 + -2.0 3,540,472 - -0.4 3,540,472 - +0.5 3,540,472 - -0.7 3,540,495 - -0.2 3,540,540 + -0.9 3,540,541 - -0.9 3,540,548 + -0.9 3,540,548 + -0.4 3,540,549 - +0.4 3,540,549 - -1.1 3,540,549 - -0.4 3,540,549 - -0.8 3,540,549 - -1.4 3,540,549 - -1.4 3,540,558 + -1.6 3,540,646 - -1.7 3,540,827 - +2.2 3,540,896 - -2.2 3,540,896 - -0.9 3,540,896 - +0.2 3,540,973 + BPHYT_RS15610 0.16 -0.7 3,540,974 - BPHYT_RS15610 0.16 +0.1 3,541,033 + BPHYT_RS15610 0.21 -2.7 3,541,034 - BPHYT_RS15610 0.21 -0.3 3,541,272 - +0.2 3,541,344 + BPHYT_RS15610 0.47 -1.3 3,541,345 - BPHYT_RS15610 0.47 +0.1 3,541,345 - BPHYT_RS15610 0.47 +0.5 3,541,438 + BPHYT_RS15610 0.55 +1.8 3,541,439 - BPHYT_RS15610 0.55 -1.3 3,541,439 - BPHYT_RS15610 0.55 +0.5 3,541,487 - BPHYT_RS15610 0.59 +0.8 3,541,513 + BPHYT_RS15610 0.61 -1.2 3,541,562 + BPHYT_RS15610 0.65 +1.9 3,541,562 + BPHYT_RS15610 0.65 -0.9 3,541,562 + BPHYT_RS15610 0.65 -2.0 3,541,562 + BPHYT_RS15610 0.65 -3.2 3,541,562 + BPHYT_RS15610 0.65 -1.0 3,541,562 + BPHYT_RS15610 0.65 +1.4 3,541,562 + BPHYT_RS15610 0.65 -0.7 3,541,562 + BPHYT_RS15610 0.65 -0.2 3,541,563 - BPHYT_RS15610 0.66 +1.9 3,541,563 - BPHYT_RS15610 0.66 -0.3 3,541,563 - BPHYT_RS15610 0.66 -1.3 3,541,564 + BPHYT_RS15610 0.66 -1.8 3,541,564 + BPHYT_RS15610 0.66 -0.9 3,541,564 + BPHYT_RS15610 0.66 -1.1 3,541,564 + BPHYT_RS15610 0.66 -0.4 3,541,565 - BPHYT_RS15610 0.66 +1.7 3,541,565 - BPHYT_RS15610 0.66 -0.9 3,541,565 - BPHYT_RS15610 0.66 -0.0 3,541,565 - BPHYT_RS15610 0.66 +0.7 3,541,565 - BPHYT_RS15610 0.66 +0.9 3,541,565 - BPHYT_RS15610 0.66 -0.7 3,541,565 - BPHYT_RS15610 0.66 -1.2 3,541,566 + BPHYT_RS15610 0.66 -0.6 3,541,566 + BPHYT_RS15610 0.66 -1.8 3,541,566 + BPHYT_RS15610 0.66 +0.4 3,541,567 - BPHYT_RS15610 0.66 -0.8 3,541,567 - +0.5 3,541,567 - BPHYT_RS15610 0.66 -0.4 3,541,977 + +0.6 3,541,978 - -0.7 3,541,978 - -1.7 3,541,981 + -0.4 3,541,982 - +0.4 3,542,007 - +0.9 3,542,022 - +1.6 3,542,070 + +1.1 3,542,070 + -0.1 3,542,070 + -1.6 3,542,071 - +0.4 3,542,251 + +0.3 3,542,252 - +0.4 3,542,255 - +0.2 3,542,291 - -1.9 3,542,291 - -1.1 3,542,319 + -0.9 3,542,350 + -0.0 3,542,350 + -0.1 3,542,350 + -1.6 3,542,350 + -0.8 3,542,350 + -0.0 3,542,356 + -0.0 3,542,356 + -0.1 3,542,409 + -0.7 3,542,409 + -1.5 3,542,410 - -0.2 3,542,728 + BPHYT_RS15615 0.22 +0.2 3,542,864 - BPHYT_RS15615 0.36 +2.5
Or see this region's nucleotide sequence