Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15380 and BPHYT_RS15385 are separated by 15 nucleotides BPHYT_RS15385 and BPHYT_RS15390 are separated by 201 nucleotides BPHYT_RS15390 and BPHYT_RS15395 are separated by 49 nucleotides BPHYT_RS15395 and BPHYT_RS15400 overlap by 4 nucleotides
BPHYT_RS15380: BPHYT_RS15380 - diaminohydroxyphosphoribosylaminopyrimidine deaminase, at 3,491,753 to 3,492,904
_RS15380
BPHYT_RS15385: BPHYT_RS15385 - riboflavin synthase subunit alpha, at 3,492,920 to 3,493,534
_RS15385
BPHYT_RS15390: BPHYT_RS15390 - 3,4-Dihydroxy-2-butanone 4-phosphate synthase, at 3,493,736 to 3,494,872
_RS15390
BPHYT_RS15395: BPHYT_RS15395 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,494,922 to 3,495,428
_RS15395
BPHYT_RS15400: BPHYT_RS15400 - N utilization substance protein B homolog, at 3,495,425 to 3,495,859
_RS15400
Position (kb)
3493
3494
3495 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3492.850 kb on + strand at 3492.913 kb on + strand at 3493.532 kb on + strand at 3493.533 kb on - strand at 3493.533 kb on - strand at 3493.552 kb on + strand at 3493.552 kb on + strand at 3493.558 kb on + strand at 3493.558 kb on + strand at 3493.558 kb on + strand at 3493.559 kb on - strand at 3493.559 kb on - strand at 3493.559 kb on - strand at 3493.621 kb on + strand at 3493.622 kb on - strand at 3493.662 kb on + strand at 3493.703 kb on - strand at 3493.707 kb on + strand at 3493.755 kb on + strand at 3493.755 kb on + strand at 3494.327 kb on + strand, within BPHYT_RS15390 at 3494.411 kb on + strand, within BPHYT_RS15390 at 3494.411 kb on + strand, within BPHYT_RS15390 at 3494.714 kb on + strand, within BPHYT_RS15390 at 3494.714 kb on + strand, within BPHYT_RS15390 at 3494.736 kb on + strand, within BPHYT_RS15390 at 3494.736 kb on + strand, within BPHYT_RS15390 at 3495.474 kb on - strand, within BPHYT_RS15400
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,492,850 + -0.6 3,492,913 + +0.9 3,493,532 + -0.2 3,493,533 - +0.1 3,493,533 - -0.6 3,493,552 + -2.1 3,493,552 + -0.8 3,493,558 + -3.1 3,493,558 + -0.3 3,493,558 + +1.1 3,493,559 - -1.2 3,493,559 - -1.3 3,493,559 - +0.1 3,493,621 + +2.7 3,493,622 - -1.2 3,493,662 + +0.4 3,493,703 - -0.3 3,493,707 + +0.1 3,493,755 + -1.0 3,493,755 + +1.9 3,494,327 + BPHYT_RS15390 0.52 -1.1 3,494,411 + BPHYT_RS15390 0.59 +1.5 3,494,411 + BPHYT_RS15390 0.59 +0.3 3,494,714 + BPHYT_RS15390 0.86 -0.7 3,494,714 + BPHYT_RS15390 0.86 +0.4 3,494,736 + BPHYT_RS15390 0.88 +0.3 3,494,736 + BPHYT_RS15390 0.88 +0.1 3,495,474 - BPHYT_RS15400 0.11 +0.9
Or see this region's nucleotide sequence