Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15170 and BPHYT_RS15175 overlap by 4 nucleotides BPHYT_RS15175 and BPHYT_RS15180 overlap by 4 nucleotides BPHYT_RS15180 and BPHYT_RS15185 overlap by 1 nucleotides BPHYT_RS15185 and BPHYT_RS15190 are separated by 3 nucleotides
BPHYT_RS15170: BPHYT_RS15170 - family 2 glycosyl transferase, at 3,451,786 to 3,453,513
_RS15170
BPHYT_RS15175: BPHYT_RS15175 - AMP-dependent synthetase, at 3,453,510 to 3,455,216
_RS15175
BPHYT_RS15180: BPHYT_RS15180 - membrane protein, at 3,455,213 to 3,455,875
_RS15180
BPHYT_RS15185: BPHYT_RS15185 - acyl carrier protein, at 3,455,875 to 3,456,126
_RS15185
BPHYT_RS15190: BPHYT_RS15190 - acyl carrier protein, at 3,456,130 to 3,456,429
_RS15190
Position (kb)
3453
3454
3455
3456 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3452.564 kb on + strand, within BPHYT_RS15170 at 3452.832 kb on + strand, within BPHYT_RS15170 at 3452.898 kb on - strand, within BPHYT_RS15170 at 3452.898 kb on - strand, within BPHYT_RS15170 at 3452.943 kb on - strand, within BPHYT_RS15170 at 3453.805 kb on + strand, within BPHYT_RS15175 at 3454.117 kb on + strand, within BPHYT_RS15175 at 3454.117 kb on + strand, within BPHYT_RS15175 at 3454.651 kb on - strand, within BPHYT_RS15175 at 3454.672 kb on + strand, within BPHYT_RS15175 at 3454.746 kb on - strand, within BPHYT_RS15175 at 3454.746 kb on - strand, within BPHYT_RS15175 at 3454.746 kb on - strand, within BPHYT_RS15175 at 3454.865 kb on + strand, within BPHYT_RS15175 at 3454.865 kb on + strand, within BPHYT_RS15175 at 3454.866 kb on - strand, within BPHYT_RS15175 at 3454.923 kb on + strand, within BPHYT_RS15175 at 3454.934 kb on - strand, within BPHYT_RS15175 at 3455.154 kb on + strand at 3455.164 kb on + strand at 3455.164 kb on + strand at 3455.164 kb on + strand at 3455.165 kb on - strand at 3455.165 kb on - strand at 3455.301 kb on + strand, within BPHYT_RS15180 at 3455.302 kb on - strand, within BPHYT_RS15180 at 3455.302 kb on - strand, within BPHYT_RS15180 at 3455.320 kb on - strand, within BPHYT_RS15180 at 3455.358 kb on + strand, within BPHYT_RS15180 at 3455.419 kb on + strand, within BPHYT_RS15180 at 3455.419 kb on + strand, within BPHYT_RS15180 at 3455.420 kb on - strand, within BPHYT_RS15180 at 3456.132 kb on - strand at 3456.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,452,564 + BPHYT_RS15170 0.45 +0.8 3,452,832 + BPHYT_RS15170 0.61 +0.8 3,452,898 - BPHYT_RS15170 0.64 -0.2 3,452,898 - BPHYT_RS15170 0.64 -0.3 3,452,943 - BPHYT_RS15170 0.67 +1.4 3,453,805 + BPHYT_RS15175 0.17 +0.4 3,454,117 + BPHYT_RS15175 0.36 +0.6 3,454,117 + BPHYT_RS15175 0.36 +0.0 3,454,651 - BPHYT_RS15175 0.67 +1.3 3,454,672 + BPHYT_RS15175 0.68 +1.7 3,454,746 - BPHYT_RS15175 0.72 +1.3 3,454,746 - BPHYT_RS15175 0.72 -0.5 3,454,746 - BPHYT_RS15175 0.72 +1.6 3,454,865 + BPHYT_RS15175 0.79 +0.8 3,454,865 + BPHYT_RS15175 0.79 +1.4 3,454,866 - BPHYT_RS15175 0.79 +0.2 3,454,923 + BPHYT_RS15175 0.83 +0.3 3,454,934 - BPHYT_RS15175 0.83 +0.9 3,455,154 + +1.1 3,455,164 + +0.5 3,455,164 + +0.5 3,455,164 + +2.9 3,455,165 - -1.2 3,455,165 - -0.2 3,455,301 + BPHYT_RS15180 0.13 +1.1 3,455,302 - BPHYT_RS15180 0.13 +1.3 3,455,302 - BPHYT_RS15180 0.13 +0.9 3,455,320 - BPHYT_RS15180 0.16 +1.5 3,455,358 + BPHYT_RS15180 0.22 +1.2 3,455,419 + BPHYT_RS15180 0.31 -0.1 3,455,419 + BPHYT_RS15180 0.31 +0.0 3,455,420 - BPHYT_RS15180 0.31 +0.0 3,456,132 - +0.2 3,456,132 - +0.6
Or see this region's nucleotide sequence