Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15095 and BPHYT_RS15100 are separated by 105 nucleotides BPHYT_RS15100 and BPHYT_RS15105 are separated by 83 nucleotides
BPHYT_RS15095: BPHYT_RS15095 - adenine deaminase, at 3,435,980 to 3,437,032
_RS15095
BPHYT_RS15100: BPHYT_RS15100 - guanine deaminase, at 3,437,138 to 3,438,511
_RS15100
BPHYT_RS15105: BPHYT_RS15105 - FAD-dependent oxidoreductase, at 3,438,595 to 3,439,839
_RS15105
Position (kb)
3437
3438
3439 Strain fitness (log2 ratio)
-1
0
1
2 at 3436.154 kb on + strand, within BPHYT_RS15095 at 3436.154 kb on + strand, within BPHYT_RS15095 at 3436.155 kb on - strand, within BPHYT_RS15095 at 3436.191 kb on - strand, within BPHYT_RS15095 at 3436.191 kb on - strand at 3436.193 kb on + strand, within BPHYT_RS15095 at 3436.193 kb on + strand, within BPHYT_RS15095 at 3436.250 kb on + strand, within BPHYT_RS15095 at 3436.251 kb on - strand, within BPHYT_RS15095 at 3436.428 kb on + strand, within BPHYT_RS15095 at 3436.428 kb on + strand, within BPHYT_RS15095 at 3436.485 kb on - strand, within BPHYT_RS15095 at 3436.625 kb on + strand, within BPHYT_RS15095 at 3436.865 kb on + strand, within BPHYT_RS15095 at 3436.880 kb on + strand, within BPHYT_RS15095 at 3436.925 kb on + strand, within BPHYT_RS15095 at 3437.306 kb on + strand, within BPHYT_RS15100 at 3437.307 kb on - strand, within BPHYT_RS15100 at 3437.307 kb on - strand, within BPHYT_RS15100 at 3437.307 kb on - strand, within BPHYT_RS15100 at 3437.462 kb on + strand, within BPHYT_RS15100 at 3437.463 kb on - strand, within BPHYT_RS15100 at 3437.570 kb on + strand at 3437.571 kb on - strand, within BPHYT_RS15100 at 3437.585 kb on - strand, within BPHYT_RS15100 at 3437.759 kb on + strand, within BPHYT_RS15100 at 3437.759 kb on + strand, within BPHYT_RS15100 at 3437.759 kb on + strand, within BPHYT_RS15100 at 3437.760 kb on - strand, within BPHYT_RS15100 at 3437.760 kb on - strand, within BPHYT_RS15100 at 3437.760 kb on - strand, within BPHYT_RS15100 at 3437.942 kb on + strand, within BPHYT_RS15100 at 3437.943 kb on - strand, within BPHYT_RS15100 at 3437.943 kb on - strand, within BPHYT_RS15100 at 3438.231 kb on - strand, within BPHYT_RS15100 at 3438.231 kb on - strand, within BPHYT_RS15100 at 3438.425 kb on + strand at 3438.426 kb on - strand at 3438.522 kb on + strand at 3438.522 kb on + strand at 3438.522 kb on + strand at 3438.534 kb on + strand at 3438.573 kb on + strand at 3438.653 kb on + strand at 3438.653 kb on + strand at 3438.654 kb on - strand at 3438.654 kb on - strand at 3438.654 kb on - strand at 3438.678 kb on - strand at 3438.678 kb on - strand at 3438.790 kb on - strand at 3438.996 kb on - strand, within BPHYT_RS15105 at 3439.492 kb on + strand, within BPHYT_RS15105 at 3439.493 kb on - strand, within BPHYT_RS15105
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,436,154 + BPHYT_RS15095 0.17 -1.7 3,436,154 + BPHYT_RS15095 0.17 -0.3 3,436,155 - BPHYT_RS15095 0.17 -0.8 3,436,191 - BPHYT_RS15095 0.20 -1.2 3,436,191 - +1.3 3,436,193 + BPHYT_RS15095 0.20 -0.2 3,436,193 + BPHYT_RS15095 0.20 -0.5 3,436,250 + BPHYT_RS15095 0.26 +0.9 3,436,251 - BPHYT_RS15095 0.26 -1.0 3,436,428 + BPHYT_RS15095 0.43 +0.2 3,436,428 + BPHYT_RS15095 0.43 +1.2 3,436,485 - BPHYT_RS15095 0.48 -1.1 3,436,625 + BPHYT_RS15095 0.61 -0.5 3,436,865 + BPHYT_RS15095 0.84 -1.6 3,436,880 + BPHYT_RS15095 0.85 +2.4 3,436,925 + BPHYT_RS15095 0.90 +0.0 3,437,306 + BPHYT_RS15100 0.12 +0.2 3,437,307 - BPHYT_RS15100 0.12 -0.9 3,437,307 - BPHYT_RS15100 0.12 -0.1 3,437,307 - BPHYT_RS15100 0.12 -0.3 3,437,462 + BPHYT_RS15100 0.24 +0.7 3,437,463 - BPHYT_RS15100 0.24 +0.5 3,437,570 + +1.3 3,437,571 - BPHYT_RS15100 0.32 -0.5 3,437,585 - BPHYT_RS15100 0.33 -0.7 3,437,759 + BPHYT_RS15100 0.45 +0.5 3,437,759 + BPHYT_RS15100 0.45 -0.1 3,437,759 + BPHYT_RS15100 0.45 +2.2 3,437,760 - BPHYT_RS15100 0.45 -0.2 3,437,760 - BPHYT_RS15100 0.45 +0.7 3,437,760 - BPHYT_RS15100 0.45 +1.1 3,437,942 + BPHYT_RS15100 0.59 -0.5 3,437,943 - BPHYT_RS15100 0.59 -0.9 3,437,943 - BPHYT_RS15100 0.59 -0.0 3,438,231 - BPHYT_RS15100 0.80 -0.1 3,438,231 - BPHYT_RS15100 0.80 +1.3 3,438,425 + -0.2 3,438,426 - +0.9 3,438,522 + +0.4 3,438,522 + -1.5 3,438,522 + +0.3 3,438,534 + +0.7 3,438,573 + +0.2 3,438,653 + -0.3 3,438,653 + +2.5 3,438,654 - +0.3 3,438,654 - -1.2 3,438,654 - +0.6 3,438,678 - -0.5 3,438,678 - -1.7 3,438,790 - +0.5 3,438,996 - BPHYT_RS15105 0.32 +1.3 3,439,492 + BPHYT_RS15105 0.72 +0.3 3,439,493 - BPHYT_RS15105 0.72 -0.7
Or see this region's nucleotide sequence