Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14995 and BPHYT_RS15000 are separated by 8 nucleotides BPHYT_RS15000 and BPHYT_RS15005 are separated by 204 nucleotides BPHYT_RS15005 and BPHYT_RS15010 are separated by 147 nucleotides
BPHYT_RS14995: BPHYT_RS14995 - formate dehydrogenase subunit alpha, at 3,408,131 to 3,411,079
_RS14995
BPHYT_RS15000: BPHYT_RS15000 - formate dehydrogenase subunit delta, at 3,411,088 to 3,411,339
_RS15000
BPHYT_RS15005: BPHYT_RS15005 - FAD-binding monooxygenase, at 3,411,544 to 3,413,268
_RS15005
BPHYT_RS15010: BPHYT_RS15010 - cation transporter, at 3,413,416 to 3,414,516
_RS15010
Position (kb)
3411
3412
3413
3414 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3410.675 kb on + strand, within BPHYT_RS14995 at 3410.914 kb on + strand at 3410.967 kb on + strand at 3410.968 kb on - strand at 3410.968 kb on - strand at 3411.338 kb on - strand at 3411.650 kb on + strand at 3411.651 kb on - strand at 3411.651 kb on - strand at 3411.671 kb on + strand at 3411.671 kb on + strand at 3411.672 kb on - strand at 3411.672 kb on - strand at 3411.672 kb on - strand at 3411.696 kb on - strand at 3411.696 kb on - strand at 3412.226 kb on + strand, within BPHYT_RS15005 at 3412.654 kb on - strand, within BPHYT_RS15005 at 3412.791 kb on - strand, within BPHYT_RS15005 at 3413.297 kb on + strand at 3413.297 kb on + strand at 3413.298 kb on - strand at 3413.302 kb on + strand at 3413.303 kb on - strand at 3413.515 kb on + strand at 3413.803 kb on - strand, within BPHYT_RS15010 at 3413.803 kb on - strand, within BPHYT_RS15010 at 3413.804 kb on + strand, within BPHYT_RS15010 at 3413.862 kb on + strand, within BPHYT_RS15010 at 3413.863 kb on - strand, within BPHYT_RS15010 at 3414.100 kb on - strand, within BPHYT_RS15010
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,410,675 + BPHYT_RS14995 0.86 +0.2 3,410,914 + +0.2 3,410,967 + -0.3 3,410,968 - +2.1 3,410,968 - -1.0 3,411,338 - -0.3 3,411,650 + -1.9 3,411,651 - +0.4 3,411,651 - -0.1 3,411,671 + -1.2 3,411,671 + +0.5 3,411,672 - -0.9 3,411,672 - -1.9 3,411,672 - -0.9 3,411,696 - -0.5 3,411,696 - -0.7 3,412,226 + BPHYT_RS15005 0.40 -0.8 3,412,654 - BPHYT_RS15005 0.64 -1.1 3,412,791 - BPHYT_RS15005 0.72 -0.3 3,413,297 + -0.2 3,413,297 + -1.4 3,413,298 - -0.4 3,413,302 + -0.9 3,413,303 - -0.6 3,413,515 + -1.5 3,413,803 - BPHYT_RS15010 0.35 +0.9 3,413,803 - BPHYT_RS15010 0.35 +0.6 3,413,804 + BPHYT_RS15010 0.35 -0.2 3,413,862 + BPHYT_RS15010 0.41 +0.1 3,413,863 - BPHYT_RS15010 0.41 +0.6 3,414,100 - BPHYT_RS15010 0.62 -1.7
Or see this region's nucleotide sequence