Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14415

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14405 and BPHYT_RS14410 are separated by 101 nucleotidesBPHYT_RS14410 and BPHYT_RS14415 are separated by 117 nucleotidesBPHYT_RS14415 and BPHYT_RS14420 are separated by 232 nucleotidesBPHYT_RS14420 and BPHYT_RS14425 are separated by 152 nucleotides BPHYT_RS14405: BPHYT_RS14405 - RNA polymerase sigma factor RpoE, at 3,287,548 to 3,288,147 _RS14405 BPHYT_RS14410: BPHYT_RS14410 - hypothetical protein, at 3,288,249 to 3,288,632 _RS14410 BPHYT_RS14415: BPHYT_RS14415 - 3-oxoacyl-ACP synthase, at 3,288,750 to 3,289,988 _RS14415 BPHYT_RS14420: BPHYT_RS14420 - acyl carrier protein, at 3,290,221 to 3,290,460 _RS14420 BPHYT_RS14425: BPHYT_RS14425 - 3-ketoacyl-ACP reductase, at 3,290,613 to 3,291,362 _RS14425 Position (kb) 3288 3289 3290Strain fitness (log2 ratio) -2 -1 0 1 2at 3288.193 kb on - strandat 3288.193 kb on - strandat 3288.218 kb on - strandat 3288.218 kb on - strandat 3288.324 kb on + strand, within BPHYT_RS14410at 3288.494 kb on - strand, within BPHYT_RS14410at 3288.546 kb on - strand, within BPHYT_RS14410at 3288.583 kb on + strand, within BPHYT_RS14410at 3290.568 kb on - strandat 3290.576 kb on - strandat 3290.598 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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3,288,193 - -0.3
3,288,193 - +1.0
3,288,218 - +0.2
3,288,218 - +0.8
3,288,324 + BPHYT_RS14410 0.20 +2.6
3,288,494 - BPHYT_RS14410 0.64 -0.1
3,288,546 - BPHYT_RS14410 0.77 +0.8
3,288,583 + BPHYT_RS14410 0.87 -0.8
3,290,568 - +0.2
3,290,576 - -1.8
3,290,598 - +0.6

Or see this region's nucleotide sequence