Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13705 and BPHYT_RS13710 are separated by 373 nucleotides BPHYT_RS13710 and BPHYT_RS13715 are separated by 279 nucleotides BPHYT_RS13715 and BPHYT_RS13720 are separated by 240 nucleotides
BPHYT_RS13705: BPHYT_RS13705 - chorismate synthase, at 3,122,861 to 3,123,961
_RS13705
BPHYT_RS13710: BPHYT_RS13710 - glycerol acyltransferase, at 3,124,335 to 3,126,272
_RS13710
BPHYT_RS13715: BPHYT_RS13715 - hypothetical protein, at 3,126,552 to 3,127,013
_RS13715
BPHYT_RS13720: BPHYT_RS13720 - O-acetylhomoserine aminocarboxypropyltransferase, at 3,127,254 to 3,128,606
_RS13720
Position (kb) 3124
3125
3126
3127 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3123.495 kb on + strand, within BPHYT_RS13705 at 3123.495 kb on + strand, within BPHYT_RS13705 at 3123.496 kb on - strand, within BPHYT_RS13705 at 3123.630 kb on + strand, within BPHYT_RS13705 at 3123.630 kb on + strand, within BPHYT_RS13705 at 3123.995 kb on - strand at 3123.995 kb on - strand at 3123.997 kb on + strand at 3123.998 kb on - strand at 3123.998 kb on - strand at 3123.998 kb on - strand at 3124.062 kb on - strand at 3124.062 kb on - strand at 3124.063 kb on + strand at 3124.063 kb on + strand at 3124.064 kb on - strand at 3124.212 kb on + strand at 3124.212 kb on + strand at 3124.707 kb on - strand, within BPHYT_RS13710 at 3124.961 kb on - strand, within BPHYT_RS13710 at 3124.966 kb on + strand, within BPHYT_RS13710 at 3124.966 kb on + strand, within BPHYT_RS13710 at 3124.966 kb on + strand, within BPHYT_RS13710 at 3125.149 kb on + strand, within BPHYT_RS13710 at 3125.149 kb on + strand, within BPHYT_RS13710 at 3125.149 kb on + strand, within BPHYT_RS13710 at 3125.304 kb on + strand, within BPHYT_RS13710 at 3125.446 kb on - strand at 3125.538 kb on - strand, within BPHYT_RS13710 at 3125.824 kb on + strand, within BPHYT_RS13710 at 3125.825 kb on - strand, within BPHYT_RS13710 at 3125.825 kb on - strand, within BPHYT_RS13710 at 3125.825 kb on - strand, within BPHYT_RS13710 at 3125.830 kb on + strand, within BPHYT_RS13710 at 3125.831 kb on - strand, within BPHYT_RS13710 at 3125.884 kb on + strand, within BPHYT_RS13710 at 3126.101 kb on - strand at 3126.599 kb on + strand, within BPHYT_RS13715 at 3126.600 kb on - strand, within BPHYT_RS13715 at 3126.601 kb on + strand, within BPHYT_RS13715 at 3126.601 kb on + strand, within BPHYT_RS13715 at 3126.601 kb on + strand, within BPHYT_RS13715 at 3126.601 kb on + strand, within BPHYT_RS13715 at 3126.602 kb on - strand, within BPHYT_RS13715 at 3126.602 kb on - strand, within BPHYT_RS13715 at 3126.661 kb on + strand, within BPHYT_RS13715 at 3126.661 kb on + strand, within BPHYT_RS13715 at 3126.886 kb on + strand, within BPHYT_RS13715 at 3126.894 kb on + strand, within BPHYT_RS13715 at 3126.993 kb on - strand at 3127.046 kb on - strand at 3127.084 kb on + strand at 3127.085 kb on - strand at 3127.085 kb on - strand at 3127.199 kb on - strand at 3127.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,123,495 + BPHYT_RS13705 0.58 -0.0 3,123,495 + BPHYT_RS13705 0.58 -1.1 3,123,496 - BPHYT_RS13705 0.58 -1.1 3,123,630 + BPHYT_RS13705 0.70 +1.0 3,123,630 + BPHYT_RS13705 0.70 +2.3 3,123,995 - -0.7 3,123,995 - -0.5 3,123,997 + -0.8 3,123,998 - -0.2 3,123,998 - +1.5 3,123,998 - +0.6 3,124,062 - -0.6 3,124,062 - -0.4 3,124,063 + -0.0 3,124,063 + +0.1 3,124,064 - +1.6 3,124,212 + -0.0 3,124,212 + -0.4 3,124,707 - BPHYT_RS13710 0.19 -0.2 3,124,961 - BPHYT_RS13710 0.32 -2.4 3,124,966 + BPHYT_RS13710 0.33 -1.0 3,124,966 + BPHYT_RS13710 0.33 -0.4 3,124,966 + BPHYT_RS13710 0.33 +1.5 3,125,149 + BPHYT_RS13710 0.42 -1.3 3,125,149 + BPHYT_RS13710 0.42 +0.2 3,125,149 + BPHYT_RS13710 0.42 -0.8 3,125,304 + BPHYT_RS13710 0.50 -1.0 3,125,446 - +1.8 3,125,538 - BPHYT_RS13710 0.62 -0.4 3,125,824 + BPHYT_RS13710 0.77 +0.4 3,125,825 - BPHYT_RS13710 0.77 -1.6 3,125,825 - BPHYT_RS13710 0.77 +0.6 3,125,825 - BPHYT_RS13710 0.77 +0.0 3,125,830 + BPHYT_RS13710 0.77 -1.6 3,125,831 - BPHYT_RS13710 0.77 +0.1 3,125,884 + BPHYT_RS13710 0.80 -0.2 3,126,101 - -0.2 3,126,599 + BPHYT_RS13715 0.10 +0.2 3,126,600 - BPHYT_RS13715 0.10 +0.3 3,126,601 + BPHYT_RS13715 0.11 +0.3 3,126,601 + BPHYT_RS13715 0.11 +1.5 3,126,601 + BPHYT_RS13715 0.11 -1.1 3,126,601 + BPHYT_RS13715 0.11 -0.6 3,126,602 - BPHYT_RS13715 0.11 +1.8 3,126,602 - BPHYT_RS13715 0.11 +0.1 3,126,661 + BPHYT_RS13715 0.24 -0.1 3,126,661 + BPHYT_RS13715 0.24 -1.5 3,126,886 + BPHYT_RS13715 0.72 -0.2 3,126,894 + BPHYT_RS13715 0.74 +0.4 3,126,993 - -0.3 3,127,046 - -0.7 3,127,084 + -0.8 3,127,085 - +0.4 3,127,085 - -0.4 3,127,199 - -0.6 3,127,199 - -0.5
Or see this region's nucleotide sequence