Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13125 and BPHYT_RS13130 are separated by 45 nucleotides BPHYT_RS13130 and BPHYT_RS13135 are separated by 10 nucleotides BPHYT_RS13135 and BPHYT_RS13140 are separated by 209 nucleotides
BPHYT_RS13125: BPHYT_RS13125 - ABC transporter ATP-binding protein, at 2,998,638 to 2,999,684
_RS13125
BPHYT_RS13130: BPHYT_RS13130 - metallophosphatase, at 2,999,730 to 3,000,557
_RS13130
BPHYT_RS13135: BPHYT_RS13135 - hypothetical protein, at 3,000,568 to 3,001,053
_RS13135
BPHYT_RS13140: BPHYT_RS13140 - MFS transporter, at 3,001,263 to 3,002,462
_RS13140
Position (kb)
2999
3000
3001 Strain fitness (log2 ratio)
-1
0
1 at 2999.218 kb on - strand, within BPHYT_RS13125 at 2999.316 kb on + strand, within BPHYT_RS13125 at 2999.316 kb on + strand, within BPHYT_RS13125 at 2999.317 kb on - strand, within BPHYT_RS13125 at 2999.487 kb on + strand, within BPHYT_RS13125 at 2999.683 kb on - strand at 2999.712 kb on + strand at 2999.713 kb on - strand at 2999.949 kb on + strand, within BPHYT_RS13130 at 3000.495 kb on + strand at 3000.495 kb on + strand at 3000.496 kb on - strand at 3000.717 kb on - strand, within BPHYT_RS13135 at 3000.967 kb on - strand, within BPHYT_RS13135 at 3000.968 kb on + strand, within BPHYT_RS13135 at 3000.969 kb on - strand, within BPHYT_RS13135 at 3001.041 kb on + strand at 3001.068 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,999,218 - BPHYT_RS13125 0.55 +1.6 2,999,316 + BPHYT_RS13125 0.65 +0.9 2,999,316 + BPHYT_RS13125 0.65 -0.6 2,999,317 - BPHYT_RS13125 0.65 -1.3 2,999,487 + BPHYT_RS13125 0.81 -0.5 2,999,683 - +0.1 2,999,712 + +0.4 2,999,713 - -0.4 2,999,949 + BPHYT_RS13130 0.26 -1.7 3,000,495 + +1.0 3,000,495 + -1.0 3,000,496 - -0.1 3,000,717 - BPHYT_RS13135 0.31 +0.1 3,000,967 - BPHYT_RS13135 0.82 +0.1 3,000,968 + BPHYT_RS13135 0.82 +1.0 3,000,969 - BPHYT_RS13135 0.83 -1.2 3,001,041 + +0.1 3,001,068 - +0.5
Or see this region's nucleotide sequence