Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11280 and BPHYT_RS11285 are separated by 71 nucleotides BPHYT_RS11285 and BPHYT_RS11290 are separated by 19 nucleotides BPHYT_RS11290 and BPHYT_RS11295 overlap by 1 nucleotides
BPHYT_RS11280: BPHYT_RS11280 - membrane protein, at 2,561,832 to 2,562,650
_RS11280
BPHYT_RS11285: BPHYT_RS11285 - LacI family transcription regulator, at 2,562,722 to 2,563,810
_RS11285
BPHYT_RS11290: BPHYT_RS11290 - 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43) (from data) , at 2,563,830 to 2,564,795
_RS11290
BPHYT_RS11295: BPHYT_RS11295 - MFS transporter, at 2,564,795 to 2,566,090
_RS11295
Position (kb)
2562
2563
2564 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2561.858 kb on - strand at 2561.902 kb on + strand at 2561.902 kb on + strand at 2561.903 kb on - strand at 2561.903 kb on - strand at 2561.903 kb on - strand at 2561.903 kb on - strand at 2561.998 kb on - strand, within BPHYT_RS11280 at 2562.406 kb on + strand, within BPHYT_RS11280 at 2562.406 kb on + strand, within BPHYT_RS11280 at 2562.469 kb on + strand, within BPHYT_RS11280 at 2562.470 kb on - strand, within BPHYT_RS11280 at 2562.588 kb on + strand at 2562.632 kb on + strand at 2562.646 kb on - strand at 2562.788 kb on + strand at 2562.799 kb on + strand at 2562.800 kb on - strand at 2563.315 kb on + strand, within BPHYT_RS11285 at 2563.437 kb on + strand, within BPHYT_RS11285 at 2563.952 kb on + strand, within BPHYT_RS11290 at 2564.288 kb on - strand, within BPHYT_RS11290 at 2564.288 kb on - strand, within BPHYT_RS11290
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,561,858 - +0.3 2,561,902 + +0.9 2,561,902 + -1.5 2,561,903 - +0.8 2,561,903 - +0.3 2,561,903 - +3.3 2,561,903 - -0.1 2,561,998 - BPHYT_RS11280 0.20 +2.5 2,562,406 + BPHYT_RS11280 0.70 -0.9 2,562,406 + BPHYT_RS11280 0.70 +0.1 2,562,469 + BPHYT_RS11280 0.78 -1.0 2,562,470 - BPHYT_RS11280 0.78 -1.2 2,562,588 + +2.5 2,562,632 + -0.9 2,562,646 - +0.8 2,562,788 + -0.8 2,562,799 + -1.8 2,562,800 - -0.6 2,563,315 + BPHYT_RS11285 0.54 -0.1 2,563,437 + BPHYT_RS11285 0.66 -0.3 2,563,952 + BPHYT_RS11290 0.13 +0.2 2,564,288 - BPHYT_RS11290 0.47 -0.8 2,564,288 - BPHYT_RS11290 0.47 -0.9
Or see this region's nucleotide sequence