Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10570 and BPHYT_RS10575 are separated by 330 nucleotides BPHYT_RS10575 and BPHYT_RS10580 overlap by 4 nucleotides
BPHYT_RS10570: BPHYT_RS10570 - LysR family transcriptional regulator, at 2,408,255 to 2,409,151
_RS10570
BPHYT_RS10575: BPHYT_RS10575 - hypothetical protein, at 2,409,482 to 2,410,060
_RS10575
BPHYT_RS10580: BPHYT_RS10580 - molecular chaperone protein HspA/DnaK, at 2,410,057 to 2,411,916
_RS10580
Position (kb)
2409
2410
2411 Strain fitness (log2 ratio)
-2
-1
0
1 at 2409.039 kb on - strand, within BPHYT_RS10570 at 2409.250 kb on + strand at 2409.251 kb on - strand at 2409.296 kb on - strand at 2409.767 kb on + strand, within BPHYT_RS10575 at 2409.768 kb on - strand, within BPHYT_RS10575 at 2409.768 kb on - strand, within BPHYT_RS10575 at 2409.768 kb on - strand, within BPHYT_RS10575 at 2409.768 kb on - strand, within BPHYT_RS10575 at 2410.056 kb on + strand at 2410.057 kb on - strand at 2410.057 kb on - strand at 2410.057 kb on - strand at 2410.081 kb on + strand at 2410.127 kb on - strand at 2410.127 kb on - strand at 2410.240 kb on + strand at 2410.241 kb on - strand at 2410.279 kb on + strand, within BPHYT_RS10580 at 2410.662 kb on + strand, within BPHYT_RS10580 at 2410.662 kb on + strand, within BPHYT_RS10580 at 2410.662 kb on + strand, within BPHYT_RS10580 at 2410.663 kb on - strand, within BPHYT_RS10580 at 2410.933 kb on + strand, within BPHYT_RS10580
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,409,039 - BPHYT_RS10570 0.87 +0.3 2,409,250 + -0.7 2,409,251 - +1.1 2,409,296 - -2.0 2,409,767 + BPHYT_RS10575 0.49 -2.6 2,409,768 - BPHYT_RS10575 0.49 -1.0 2,409,768 - BPHYT_RS10575 0.49 -1.2 2,409,768 - BPHYT_RS10575 0.49 -0.5 2,409,768 - BPHYT_RS10575 0.49 -1.2 2,410,056 + -1.2 2,410,057 - -0.2 2,410,057 - +0.7 2,410,057 - -1.4 2,410,081 + +0.3 2,410,127 - +0.3 2,410,127 - +0.4 2,410,240 + -0.2 2,410,241 - -0.7 2,410,279 + BPHYT_RS10580 0.12 -0.0 2,410,662 + BPHYT_RS10580 0.33 +1.2 2,410,662 + BPHYT_RS10580 0.33 -0.0 2,410,662 + BPHYT_RS10580 0.33 -0.9 2,410,663 - BPHYT_RS10580 0.33 +0.6 2,410,933 + BPHYT_RS10580 0.47 -0.3
Or see this region's nucleotide sequence