Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10540

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10535 and BPHYT_RS10540 overlap by 4 nucleotidesBPHYT_RS10540 and BPHYT_RS10545 are separated by 278 nucleotides BPHYT_RS10535: BPHYT_RS10535 - DSBA oxidoreductase, at 2,399,906 to 2,400,982 _RS10535 BPHYT_RS10540: BPHYT_RS10540 - secretion protein, at 2,400,979 to 2,402,520 _RS10540 BPHYT_RS10545: BPHYT_RS10545 - fructose-1,6-bisphosphate aldolase, at 2,402,799 to 2,403,695 _RS10545 Position (kb) 2400 2401 2402 2403Strain fitness (log2 ratio) -1 0 1at 2400.036 kb on - strand, within BPHYT_RS10535at 2400.221 kb on + strand, within BPHYT_RS10535at 2400.656 kb on + strand, within BPHYT_RS10535at 2400.718 kb on + strand, within BPHYT_RS10535at 2401.325 kb on - strand, within BPHYT_RS10540at 2401.520 kb on + strandat 2401.564 kb on + strand, within BPHYT_RS10540

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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2,400,036 - BPHYT_RS10535 0.12 -0.7
2,400,221 + BPHYT_RS10535 0.29 -0.5
2,400,656 + BPHYT_RS10535 0.70 +0.3
2,400,718 + BPHYT_RS10535 0.75 -1.6
2,401,325 - BPHYT_RS10540 0.22 -0.8
2,401,520 + +1.2
2,401,564 + BPHYT_RS10540 0.38 -1.1

Or see this region's nucleotide sequence