Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10305

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10300 and BPHYT_RS10305 are separated by 995 nucleotidesBPHYT_RS10305 and BPHYT_RS10310 are separated by 473 nucleotides BPHYT_RS10300: BPHYT_RS10300 - racemase, at 2,341,977 to 2,342,714 _RS10300 BPHYT_RS10305: BPHYT_RS10305 - hypothetical protein, at 2,343,710 to 2,343,898 _RS10305 BPHYT_RS10310: BPHYT_RS10310 - catalase-peroxidase, at 2,344,372 to 2,346,633 _RS10310 Position (kb) 2343 2344Strain fitness (log2 ratio) -2 -1 0 1 2at 2342.755 kb on - strandat 2343.284 kb on + strandat 2343.381 kb on + strandat 2343.381 kb on + strandat 2343.386 kb on - strandat 2343.386 kb on - strandat 2343.685 kb on + strandat 2343.852 kb on - strand, within BPHYT_RS10305at 2344.070 kb on - strandat 2344.078 kb on - strandat 2344.097 kb on + strandat 2344.109 kb on - strandat 2344.167 kb on - strandat 2344.224 kb on + strandat 2344.224 kb on + strandat 2344.245 kb on + strandat 2344.246 kb on - strandat 2344.246 kb on - strandat 2344.313 kb on - strandat 2344.348 kb on - strandat 2344.660 kb on + strand, within BPHYT_RS10310at 2344.660 kb on + strand, within BPHYT_RS10310at 2344.661 kb on - strand, within BPHYT_RS10310at 2344.787 kb on - strand, within BPHYT_RS10310

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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2,342,755 - -0.3
2,343,284 + -0.3
2,343,381 + +0.2
2,343,381 + +2.2
2,343,386 - +1.2
2,343,386 - +0.2
2,343,685 + +1.2
2,343,852 - BPHYT_RS10305 0.75 -1.9
2,344,070 - -0.3
2,344,078 - -0.6
2,344,097 + -0.6
2,344,109 - +1.0
2,344,167 - +0.2
2,344,224 + +1.2
2,344,224 + -0.7
2,344,245 + +1.3
2,344,246 - +0.2
2,344,246 - +1.2
2,344,313 - +0.6
2,344,348 - -2.1
2,344,660 + BPHYT_RS10310 0.13 +0.2
2,344,660 + BPHYT_RS10310 0.13 -1.1
2,344,661 - BPHYT_RS10310 0.13 +0.2
2,344,787 - BPHYT_RS10310 0.18 +0.2

Or see this region's nucleotide sequence