Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10115 and BPHYT_RS10120 are separated by 275 nucleotides BPHYT_RS10120 and BPHYT_RS10125 are separated by 160 nucleotides BPHYT_RS10125 and BPHYT_RS10130 are separated by 711 nucleotides BPHYT_RS10130 and BPHYT_RS10135 are separated by 35 nucleotides
BPHYT_RS10115: BPHYT_RS10115 - hypothetical protein, at 2,304,178 to 2,304,921
_RS10115
BPHYT_RS10120: BPHYT_RS10120 - membrane protein, at 2,305,197 to 2,305,748
_RS10120
BPHYT_RS10125: BPHYT_RS10125 - hypothetical protein, at 2,305,909 to 2,306,361
_RS10125
BPHYT_RS10130: BPHYT_RS10130 - hypothetical protein, at 2,307,073 to 2,307,252
_RS10130
BPHYT_RS10135: BPHYT_RS10135 - hypothetical protein, at 2,307,288 to 2,307,713
_RS10135
Position (kb)
2305
2306
2307 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2304.998 kb on + strand at 2304.998 kb on + strand at 2304.999 kb on - strand at 2305.102 kb on + strand at 2305.102 kb on + strand at 2305.196 kb on + strand at 2305.261 kb on + strand, within BPHYT_RS10120 at 2305.262 kb on - strand, within BPHYT_RS10120 at 2305.262 kb on - strand, within BPHYT_RS10120 at 2305.262 kb on - strand, within BPHYT_RS10120 at 2305.806 kb on + strand at 2306.194 kb on + strand, within BPHYT_RS10125 at 2306.478 kb on - strand at 2306.478 kb on - strand at 2306.702 kb on - strand at 2306.846 kb on + strand at 2307.012 kb on + strand at 2307.031 kb on - strand at 2307.119 kb on + strand, within BPHYT_RS10130 at 2307.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,304,998 + -0.3 2,304,998 + +0.4 2,304,999 - -2.1 2,305,102 + +0.5 2,305,102 + -0.1 2,305,196 + -0.0 2,305,261 + BPHYT_RS10120 0.12 -0.1 2,305,262 - BPHYT_RS10120 0.12 +1.7 2,305,262 - BPHYT_RS10120 0.12 -0.1 2,305,262 - BPHYT_RS10120 0.12 +1.9 2,305,806 + -1.4 2,306,194 + BPHYT_RS10125 0.63 -1.6 2,306,478 - -1.6 2,306,478 - -1.4 2,306,702 - +1.0 2,306,846 + -0.3 2,307,012 + +0.0 2,307,031 - +1.1 2,307,119 + BPHYT_RS10130 0.26 +2.1 2,307,247 - +0.1
Or see this region's nucleotide sequence