Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09055 and BPHYT_RS09060 are separated by 67 nucleotides BPHYT_RS09060 and BPHYT_RS09065 overlap by 17 nucleotides BPHYT_RS09065 and BPHYT_RS09070 are separated by 23 nucleotides BPHYT_RS09070 and BPHYT_RS09075 are separated by 236 nucleotides
BPHYT_RS09055: BPHYT_RS09055 - DNA polymerase III subunit gamma/tau, at 2,049,245 to 2,051,926
_RS09055
BPHYT_RS09060: BPHYT_RS09060 - hypothetical protein, at 2,051,994 to 2,052,320
_RS09060
BPHYT_RS09065: BPHYT_RS09065 - hypothetical protein, at 2,052,304 to 2,052,570
_RS09065
BPHYT_RS09070: BPHYT_RS09070 - recombination protein RecR, at 2,052,594 to 2,053,190
_RS09070
BPHYT_RS09075: BPHYT_RS09075 - CoA transferase, at 2,053,427 to 2,054,668
_RS09075
Position (kb)
2052
2053
2054 Strain fitness (log2 ratio)
-2
-1
0
1 at 2051.884 kb on + strand at 2052.571 kb on + strand at 2052.903 kb on + strand, within BPHYT_RS09070 at 2052.903 kb on + strand, within BPHYT_RS09070 at 2052.904 kb on - strand, within BPHYT_RS09070 at 2053.056 kb on + strand, within BPHYT_RS09070 at 2053.057 kb on - strand, within BPHYT_RS09070 at 2053.057 kb on - strand, within BPHYT_RS09070 at 2053.137 kb on + strand at 2053.220 kb on + strand at 2053.220 kb on + strand at 2053.221 kb on - strand at 2053.252 kb on + strand at 2053.252 kb on + strand at 2053.253 kb on - strand at 2053.253 kb on - strand at 2053.313 kb on + strand at 2053.402 kb on + strand at 2053.472 kb on - strand at 2053.930 kb on + strand, within BPHYT_RS09075 at 2053.931 kb on - strand, within BPHYT_RS09075 at 2053.931 kb on - strand, within BPHYT_RS09075 at 2054.081 kb on + strand, within BPHYT_RS09075 at 2054.124 kb on - strand, within BPHYT_RS09075 at 2054.132 kb on + strand, within BPHYT_RS09075 at 2054.132 kb on + strand, within BPHYT_RS09075 at 2054.133 kb on - strand, within BPHYT_RS09075 at 2054.133 kb on - strand, within BPHYT_RS09075 at 2054.186 kb on - strand, within BPHYT_RS09075 at 2054.188 kb on + strand, within BPHYT_RS09075 at 2054.188 kb on + strand, within BPHYT_RS09075 at 2054.189 kb on - strand, within BPHYT_RS09075
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,051,884 + -1.4 2,052,571 + +0.3 2,052,903 + BPHYT_RS09070 0.52 -0.6 2,052,903 + BPHYT_RS09070 0.52 -1.5 2,052,904 - BPHYT_RS09070 0.52 -0.6 2,053,056 + BPHYT_RS09070 0.77 -1.4 2,053,057 - BPHYT_RS09070 0.78 -0.6 2,053,057 - BPHYT_RS09070 0.78 -0.5 2,053,137 + -0.9 2,053,220 + +0.7 2,053,220 + -1.0 2,053,221 - -0.5 2,053,252 + +0.2 2,053,252 + -1.2 2,053,253 - +0.5 2,053,253 - +0.3 2,053,313 + -0.6 2,053,402 + -0.3 2,053,472 - +0.5 2,053,930 + BPHYT_RS09075 0.40 -0.8 2,053,931 - BPHYT_RS09075 0.41 +0.7 2,053,931 - BPHYT_RS09075 0.41 -0.0 2,054,081 + BPHYT_RS09075 0.53 -0.7 2,054,124 - BPHYT_RS09075 0.56 -0.0 2,054,132 + BPHYT_RS09075 0.57 +0.4 2,054,132 + BPHYT_RS09075 0.57 +0.3 2,054,133 - BPHYT_RS09075 0.57 +0.3 2,054,133 - BPHYT_RS09075 0.57 -0.9 2,054,186 - BPHYT_RS09075 0.61 -2.1 2,054,188 + BPHYT_RS09075 0.61 +0.5 2,054,188 + BPHYT_RS09075 0.61 -2.0 2,054,189 - BPHYT_RS09075 0.61 +0.7
Or see this region's nucleotide sequence