Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08885

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08875 and BPHYT_RS08880 are separated by 139 nucleotidesBPHYT_RS08880 and BPHYT_RS08885 overlap by 1 nucleotidesBPHYT_RS08885 and BPHYT_RS08890 are separated by 58 nucleotides BPHYT_RS08875: BPHYT_RS08875 - DNA helicase, at 2,008,491 to 2,009,876 _RS08875 BPHYT_RS08880: BPHYT_RS08880 - phosphate transport regulator, at 2,010,016 to 2,010,642 _RS08880 BPHYT_RS08885: BPHYT_RS08885 - inorganic phosphate transporter, at 2,010,642 to 2,011,652 _RS08885 BPHYT_RS08890: BPHYT_RS08890 - 3-oxoacyl-ACP reductase, at 2,011,711 to 2,012,517 _RS08890 Position (kb) 2010 2011 2012Strain fitness (log2 ratio) -1 0 1 2 3at 2009.924 kb on - strandat 2009.924 kb on - strandat 2009.964 kb on - strandat 2009.980 kb on - strandat 2009.980 kb on - strandat 2009.980 kb on - strandat 2009.991 kb on - strandat 2009.991 kb on - strandat 2009.991 kb on - strandat 2009.991 kb on - strandat 2010.144 kb on + strand, within BPHYT_RS08880at 2010.144 kb on + strand, within BPHYT_RS08880at 2010.319 kb on + strand, within BPHYT_RS08880at 2010.320 kb on - strand, within BPHYT_RS08880at 2010.363 kb on + strand, within BPHYT_RS08880at 2010.364 kb on - strand, within BPHYT_RS08880at 2010.563 kb on - strand, within BPHYT_RS08880at 2010.602 kb on + strandat 2010.603 kb on - strandat 2010.603 kb on - strandat 2010.641 kb on - strandat 2010.652 kb on + strandat 2010.653 kb on - strandat 2010.891 kb on + strand, within BPHYT_RS08885at 2010.949 kb on - strand, within BPHYT_RS08885at 2011.200 kb on + strand, within BPHYT_RS08885at 2011.201 kb on - strand, within BPHYT_RS08885at 2011.201 kb on - strand, within BPHYT_RS08885at 2011.201 kb on - strand, within BPHYT_RS08885at 2011.201 kb on - strand, within BPHYT_RS08885at 2011.248 kb on - strand, within BPHYT_RS08885at 2011.326 kb on + strand, within BPHYT_RS08885at 2011.651 kb on - strandat 2011.763 kb on + strandat 2011.763 kb on + strandat 2011.764 kb on - strandat 2011.764 kb on - strandat 2011.764 kb on - strandat 2011.764 kb on - strandat 2011.917 kb on + strand, within BPHYT_RS08890at 2011.976 kb on + strand, within BPHYT_RS08890at 2011.980 kb on - strand, within BPHYT_RS08890at 2012.037 kb on + strand, within BPHYT_RS08890at 2012.038 kb on - strand, within BPHYT_RS08890at 2012.156 kb on + strand, within BPHYT_RS08890at 2012.156 kb on + strand, within BPHYT_RS08890at 2012.156 kb on + strand, within BPHYT_RS08890at 2012.157 kb on - strand, within BPHYT_RS08890at 2012.157 kb on - strand, within BPHYT_RS08890at 2012.162 kb on + strand, within BPHYT_RS08890at 2012.162 kb on + strand, within BPHYT_RS08890at 2012.163 kb on - strand, within BPHYT_RS08890at 2012.172 kb on - strand, within BPHYT_RS08890at 2012.257 kb on + strand, within BPHYT_RS08890at 2012.257 kb on + strand, within BPHYT_RS08890at 2012.257 kb on + strand, within BPHYT_RS08890at 2012.258 kb on - strand, within BPHYT_RS08890at 2012.258 kb on - strand, within BPHYT_RS08890at 2012.554 kb on + strandat 2012.554 kb on + strandat 2012.555 kb on - strandat 2012.555 kb on - strandat 2012.593 kb on - strandat 2012.644 kb on + strandat 2012.645 kb on - strandat 2012.645 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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2,009,924 - +0.8
2,009,924 - +0.5
2,009,964 - +1.1
2,009,980 - +0.4
2,009,980 - -0.3
2,009,980 - +1.4
2,009,991 - +0.6
2,009,991 - +0.2
2,009,991 - +0.4
2,009,991 - +1.6
2,010,144 + BPHYT_RS08880 0.20 +1.2
2,010,144 + BPHYT_RS08880 0.20 +0.6
2,010,319 + BPHYT_RS08880 0.48 +1.1
2,010,320 - BPHYT_RS08880 0.48 +0.4
2,010,363 + BPHYT_RS08880 0.55 +0.7
2,010,364 - BPHYT_RS08880 0.56 +0.4
2,010,563 - BPHYT_RS08880 0.87 -0.9
2,010,602 + -0.1
2,010,603 - +2.3
2,010,603 - +0.6
2,010,641 - +2.4
2,010,652 + +2.1
2,010,653 - +0.9
2,010,891 + BPHYT_RS08885 0.25 +0.9
2,010,949 - BPHYT_RS08885 0.30 +1.2
2,011,200 + BPHYT_RS08885 0.55 +1.2
2,011,201 - BPHYT_RS08885 0.55 +0.5
2,011,201 - BPHYT_RS08885 0.55 +1.0
2,011,201 - BPHYT_RS08885 0.55 -0.7
2,011,201 - BPHYT_RS08885 0.55 +3.1
2,011,248 - BPHYT_RS08885 0.60 +2.2
2,011,326 + BPHYT_RS08885 0.68 -0.2
2,011,651 - +1.2
2,011,763 + -0.1
2,011,763 + -1.4
2,011,764 - -0.5
2,011,764 - +1.4
2,011,764 - -0.1
2,011,764 - +0.2
2,011,917 + BPHYT_RS08890 0.26 +0.2
2,011,976 + BPHYT_RS08890 0.33 +0.7
2,011,980 - BPHYT_RS08890 0.33 -0.1
2,012,037 + BPHYT_RS08890 0.40 -0.1
2,012,038 - BPHYT_RS08890 0.41 +0.6
2,012,156 + BPHYT_RS08890 0.55 -1.5
2,012,156 + BPHYT_RS08890 0.55 +1.2
2,012,156 + BPHYT_RS08890 0.55 -0.3
2,012,157 - BPHYT_RS08890 0.55 -0.3
2,012,157 - BPHYT_RS08890 0.55 +0.5
2,012,162 + BPHYT_RS08890 0.56 -0.2
2,012,162 + BPHYT_RS08890 0.56 +1.5
2,012,163 - BPHYT_RS08890 0.56 -0.6
2,012,172 - BPHYT_RS08890 0.57 +1.2
2,012,257 + BPHYT_RS08890 0.68 +0.4
2,012,257 + BPHYT_RS08890 0.68 +0.9
2,012,257 + BPHYT_RS08890 0.68 -0.2
2,012,258 - BPHYT_RS08890 0.68 -0.4
2,012,258 - BPHYT_RS08890 0.68 -0.2
2,012,554 + +0.3
2,012,554 + -1.1
2,012,555 - -0.3
2,012,555 - -0.8
2,012,593 - +0.5
2,012,644 + +0.1
2,012,645 - -0.9
2,012,645 - +1.8

Or see this region's nucleotide sequence