Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08875 and BPHYT_RS08880 are separated by 139 nucleotides BPHYT_RS08880 and BPHYT_RS08885 overlap by 1 nucleotides BPHYT_RS08885 and BPHYT_RS08890 are separated by 58 nucleotides
BPHYT_RS08875: BPHYT_RS08875 - DNA helicase, at 2,008,491 to 2,009,876
_RS08875
BPHYT_RS08880: BPHYT_RS08880 - phosphate transport regulator, at 2,010,016 to 2,010,642
_RS08880
BPHYT_RS08885: BPHYT_RS08885 - inorganic phosphate transporter, at 2,010,642 to 2,011,652
_RS08885
BPHYT_RS08890: BPHYT_RS08890 - 3-oxoacyl-ACP reductase, at 2,011,711 to 2,012,517
_RS08890
Position (kb)
2010
2011
2012 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2009.924 kb on - strand at 2009.924 kb on - strand at 2009.964 kb on - strand at 2009.980 kb on - strand at 2009.980 kb on - strand at 2009.980 kb on - strand at 2009.991 kb on - strand at 2009.991 kb on - strand at 2009.991 kb on - strand at 2009.991 kb on - strand at 2010.144 kb on + strand, within BPHYT_RS08880 at 2010.144 kb on + strand, within BPHYT_RS08880 at 2010.319 kb on + strand, within BPHYT_RS08880 at 2010.320 kb on - strand, within BPHYT_RS08880 at 2010.363 kb on + strand, within BPHYT_RS08880 at 2010.364 kb on - strand, within BPHYT_RS08880 at 2010.563 kb on - strand, within BPHYT_RS08880 at 2010.602 kb on + strand at 2010.603 kb on - strand at 2010.603 kb on - strand at 2010.641 kb on - strand at 2010.652 kb on + strand at 2010.653 kb on - strand at 2010.891 kb on + strand, within BPHYT_RS08885 at 2010.949 kb on - strand, within BPHYT_RS08885 at 2011.200 kb on + strand, within BPHYT_RS08885 at 2011.201 kb on - strand, within BPHYT_RS08885 at 2011.201 kb on - strand, within BPHYT_RS08885 at 2011.201 kb on - strand, within BPHYT_RS08885 at 2011.201 kb on - strand, within BPHYT_RS08885 at 2011.248 kb on - strand, within BPHYT_RS08885 at 2011.326 kb on + strand, within BPHYT_RS08885 at 2011.651 kb on - strand at 2011.763 kb on + strand at 2011.763 kb on + strand at 2011.764 kb on - strand at 2011.764 kb on - strand at 2011.764 kb on - strand at 2011.764 kb on - strand at 2011.917 kb on + strand, within BPHYT_RS08890 at 2011.976 kb on + strand, within BPHYT_RS08890 at 2011.980 kb on - strand, within BPHYT_RS08890 at 2012.037 kb on + strand, within BPHYT_RS08890 at 2012.038 kb on - strand, within BPHYT_RS08890 at 2012.156 kb on + strand, within BPHYT_RS08890 at 2012.156 kb on + strand, within BPHYT_RS08890 at 2012.156 kb on + strand, within BPHYT_RS08890 at 2012.157 kb on - strand, within BPHYT_RS08890 at 2012.157 kb on - strand, within BPHYT_RS08890 at 2012.162 kb on + strand, within BPHYT_RS08890 at 2012.162 kb on + strand, within BPHYT_RS08890 at 2012.163 kb on - strand, within BPHYT_RS08890 at 2012.172 kb on - strand, within BPHYT_RS08890 at 2012.257 kb on + strand, within BPHYT_RS08890 at 2012.257 kb on + strand, within BPHYT_RS08890 at 2012.257 kb on + strand, within BPHYT_RS08890 at 2012.258 kb on - strand, within BPHYT_RS08890 at 2012.258 kb on - strand, within BPHYT_RS08890 at 2012.554 kb on + strand at 2012.554 kb on + strand at 2012.555 kb on - strand at 2012.555 kb on - strand at 2012.593 kb on - strand at 2012.644 kb on + strand at 2012.645 kb on - strand at 2012.645 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,009,924 - +0.8 2,009,924 - +0.5 2,009,964 - +1.1 2,009,980 - +0.4 2,009,980 - -0.3 2,009,980 - +1.4 2,009,991 - +0.6 2,009,991 - +0.2 2,009,991 - +0.4 2,009,991 - +1.6 2,010,144 + BPHYT_RS08880 0.20 +1.2 2,010,144 + BPHYT_RS08880 0.20 +0.6 2,010,319 + BPHYT_RS08880 0.48 +1.1 2,010,320 - BPHYT_RS08880 0.48 +0.4 2,010,363 + BPHYT_RS08880 0.55 +0.7 2,010,364 - BPHYT_RS08880 0.56 +0.4 2,010,563 - BPHYT_RS08880 0.87 -0.9 2,010,602 + -0.1 2,010,603 - +2.3 2,010,603 - +0.6 2,010,641 - +2.4 2,010,652 + +2.1 2,010,653 - +0.9 2,010,891 + BPHYT_RS08885 0.25 +0.9 2,010,949 - BPHYT_RS08885 0.30 +1.2 2,011,200 + BPHYT_RS08885 0.55 +1.2 2,011,201 - BPHYT_RS08885 0.55 +0.5 2,011,201 - BPHYT_RS08885 0.55 +1.0 2,011,201 - BPHYT_RS08885 0.55 -0.7 2,011,201 - BPHYT_RS08885 0.55 +3.1 2,011,248 - BPHYT_RS08885 0.60 +2.2 2,011,326 + BPHYT_RS08885 0.68 -0.2 2,011,651 - +1.2 2,011,763 + -0.1 2,011,763 + -1.4 2,011,764 - -0.5 2,011,764 - +1.4 2,011,764 - -0.1 2,011,764 - +0.2 2,011,917 + BPHYT_RS08890 0.26 +0.2 2,011,976 + BPHYT_RS08890 0.33 +0.7 2,011,980 - BPHYT_RS08890 0.33 -0.1 2,012,037 + BPHYT_RS08890 0.40 -0.1 2,012,038 - BPHYT_RS08890 0.41 +0.6 2,012,156 + BPHYT_RS08890 0.55 -1.5 2,012,156 + BPHYT_RS08890 0.55 +1.2 2,012,156 + BPHYT_RS08890 0.55 -0.3 2,012,157 - BPHYT_RS08890 0.55 -0.3 2,012,157 - BPHYT_RS08890 0.55 +0.5 2,012,162 + BPHYT_RS08890 0.56 -0.2 2,012,162 + BPHYT_RS08890 0.56 +1.5 2,012,163 - BPHYT_RS08890 0.56 -0.6 2,012,172 - BPHYT_RS08890 0.57 +1.2 2,012,257 + BPHYT_RS08890 0.68 +0.4 2,012,257 + BPHYT_RS08890 0.68 +0.9 2,012,257 + BPHYT_RS08890 0.68 -0.2 2,012,258 - BPHYT_RS08890 0.68 -0.4 2,012,258 - BPHYT_RS08890 0.68 -0.2 2,012,554 + +0.3 2,012,554 + -1.1 2,012,555 - -0.3 2,012,555 - -0.8 2,012,593 - +0.5 2,012,644 + +0.1 2,012,645 - -0.9 2,012,645 - +1.8
Or see this region's nucleotide sequence