Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08530

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08525 and BPHYT_RS08530 are separated by 103 nucleotidesBPHYT_RS08530 and BPHYT_RS08535 overlap by 26 nucleotidesBPHYT_RS08535 and BPHYT_RS08540 are separated by 334 nucleotides BPHYT_RS08525: BPHYT_RS08525 - SAM-dependent methyltransferase, at 1,950,418 to 1,951,251 _RS08525 BPHYT_RS08530: BPHYT_RS08530 - mannose-1-phosphate guanylyltransferase (GDP), at 1,951,355 to 1,952,572 _RS08530 BPHYT_RS08535: BPHYT_RS08535 - flavin reductase, at 1,952,547 to 1,953,137 _RS08535 BPHYT_RS08540: BPHYT_RS08540 - GntR family transcriptional regulator, at 1,953,472 to 1,954,197 _RS08540 Position (kb) 1951 1952 1953Strain fitness (log2 ratio) -1 0 1at 1950.367 kb on + strandat 1950.367 kb on + strandat 1950.368 kb on - strandat 1950.368 kb on - strandat 1950.399 kb on - strandat 1950.746 kb on - strand, within BPHYT_RS08525at 1951.369 kb on - strandat 1952.403 kb on - strand, within BPHYT_RS08530at 1952.561 kb on - strandat 1953.380 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,950,367 + +0.8
1,950,367 + -0.4
1,950,368 - -0.0
1,950,368 - +1.2
1,950,399 - +0.6
1,950,746 - BPHYT_RS08525 0.39 -1.7
1,951,369 - -0.3
1,952,403 - BPHYT_RS08530 0.86 -1.2
1,952,561 - +0.3
1,953,380 + +0.5

Or see this region's nucleotide sequence