Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08525

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08520 and BPHYT_RS08525 are separated by 440 nucleotidesBPHYT_RS08525 and BPHYT_RS08530 are separated by 103 nucleotides BPHYT_RS08520: BPHYT_RS08520 - GNAT family acetyltransferase, at 1,949,444 to 1,949,977 _RS08520 BPHYT_RS08525: BPHYT_RS08525 - SAM-dependent methyltransferase, at 1,950,418 to 1,951,251 _RS08525 BPHYT_RS08530: BPHYT_RS08530 - mannose-1-phosphate guanylyltransferase (GDP), at 1,951,355 to 1,952,572 _RS08530 Position (kb) 1950 1951 1952Strain fitness (log2 ratio) -1 0 1at 1950.162 kb on - strandat 1950.367 kb on + strandat 1950.367 kb on + strandat 1950.368 kb on - strandat 1950.368 kb on - strandat 1950.399 kb on - strandat 1950.746 kb on - strand, within BPHYT_RS08525at 1951.369 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,950,162 - +1.4
1,950,367 + +0.8
1,950,367 + -0.4
1,950,368 - -0.0
1,950,368 - +1.2
1,950,399 - +0.6
1,950,746 - BPHYT_RS08525 0.39 -1.7
1,951,369 - -0.3

Or see this region's nucleotide sequence