Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS07415

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS07410 and BPHYT_RS07415 are separated by 391 nucleotidesBPHYT_RS07415 and BPHYT_RS07420 overlap by 1 nucleotidesBPHYT_RS07420 and BPHYT_RS07425 are separated by 294 nucleotides BPHYT_RS07410: BPHYT_RS07410 - glucosamine--fructose-6-phosphate aminotransferase, at 1,684,887 to 1,686,704 _RS07410 BPHYT_RS07415: BPHYT_RS07415 - hypothetical protein, at 1,687,096 to 1,687,419 _RS07415 BPHYT_RS07420: BPHYT_RS07420 - hypothetical protein, at 1,687,419 to 1,687,637 _RS07420 BPHYT_RS07425: BPHYT_RS07425 - hypothetical protein, at 1,687,932 to 1,688,723 _RS07425 Position (kb) 1687 1688Strain fitness (log2 ratio) -2 -1 0 1at 1686.349 kb on - strandat 1686.507 kb on + strand, within BPHYT_RS07410at 1686.508 kb on - strand, within BPHYT_RS07410at 1687.059 kb on - strandat 1687.138 kb on + strand, within BPHYT_RS07415at 1687.138 kb on + strand, within BPHYT_RS07415at 1687.138 kb on + strand, within BPHYT_RS07415at 1687.139 kb on - strand, within BPHYT_RS07415at 1687.435 kb on - strandat 1687.572 kb on - strand, within BPHYT_RS07420at 1688.010 kb on - strandat 1688.099 kb on - strand, within BPHYT_RS07425at 1688.360 kb on - strand, within BPHYT_RS07425

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,686,349 - +1.0
1,686,507 + BPHYT_RS07410 0.89 -1.8
1,686,508 - BPHYT_RS07410 0.89 +0.4
1,687,059 - +0.7
1,687,138 + BPHYT_RS07415 0.13 -0.6
1,687,138 + BPHYT_RS07415 0.13 +0.8
1,687,138 + BPHYT_RS07415 0.13 +1.5
1,687,139 - BPHYT_RS07415 0.13 +0.5
1,687,435 - +0.3
1,687,572 - BPHYT_RS07420 0.70 +0.4
1,688,010 - -1.6
1,688,099 - BPHYT_RS07425 0.21 +0.2
1,688,360 - BPHYT_RS07425 0.54 +0.7

Or see this region's nucleotide sequence