Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05430 and BPHYT_RS05435 are separated by 417 nucleotides BPHYT_RS05435 and BPHYT_RS05440 are separated by 245 nucleotides BPHYT_RS05440 and BPHYT_RS05445 overlap by 2 nucleotides
BPHYT_RS05430: BPHYT_RS05430 - GntR family transcriptional regulator, at 1,247,214 to 1,248,629
_RS05430
BPHYT_RS05435: BPHYT_RS05435 - ABC transporter substrate-binding protein, at 1,249,047 to 1,250,210
_RS05435
BPHYT_RS05440: BPHYT_RS05440 - hypothetical protein, at 1,250,456 to 1,250,650
_RS05440
BPHYT_RS05445: BPHYT_RS05445 - hypothetical protein, at 1,250,649 to 1,250,831
_RS05445
Position (kb)
1249
1250
1251 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1248.047 kb on - strand, within BPHYT_RS05430 at 1248.391 kb on + strand, within BPHYT_RS05430 at 1248.391 kb on + strand, within BPHYT_RS05430 at 1248.445 kb on + strand, within BPHYT_RS05430 at 1248.779 kb on - strand at 1248.965 kb on + strand at 1248.999 kb on + strand at 1249.261 kb on + strand, within BPHYT_RS05435 at 1249.271 kb on + strand, within BPHYT_RS05435 at 1249.321 kb on + strand, within BPHYT_RS05435 at 1249.542 kb on + strand, within BPHYT_RS05435 at 1249.566 kb on - strand, within BPHYT_RS05435 at 1249.877 kb on + strand, within BPHYT_RS05435 at 1249.993 kb on - strand, within BPHYT_RS05435 at 1250.151 kb on + strand at 1250.389 kb on - strand at 1250.611 kb on + strand, within BPHYT_RS05440 at 1250.611 kb on + strand, within BPHYT_RS05440 at 1250.632 kb on - strand at 1250.633 kb on + strand at 1250.634 kb on - strand at 1250.872 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,248,047 - BPHYT_RS05430 0.59 +1.0 1,248,391 + BPHYT_RS05430 0.83 -2.5 1,248,391 + BPHYT_RS05430 0.83 -0.4 1,248,445 + BPHYT_RS05430 0.87 -1.3 1,248,779 - +0.8 1,248,965 + -0.2 1,248,999 + +0.6 1,249,261 + BPHYT_RS05435 0.18 -0.5 1,249,271 + BPHYT_RS05435 0.19 -1.3 1,249,321 + BPHYT_RS05435 0.24 -1.3 1,249,542 + BPHYT_RS05435 0.43 -1.5 1,249,566 - BPHYT_RS05435 0.45 -0.7 1,249,877 + BPHYT_RS05435 0.71 -1.9 1,249,993 - BPHYT_RS05435 0.81 -0.2 1,250,151 + +0.8 1,250,389 - -0.5 1,250,611 + BPHYT_RS05440 0.79 -1.2 1,250,611 + BPHYT_RS05440 0.79 -4.1 1,250,632 - -0.4 1,250,633 + -0.7 1,250,634 - +0.3 1,250,872 + -0.3
Or see this region's nucleotide sequence