Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05360 and BPHYT_RS05365 are separated by 155 nucleotides BPHYT_RS05365 and BPHYT_RS05370 are separated by 43 nucleotides BPHYT_RS05370 and BPHYT_RS05375 are separated by 253 nucleotides BPHYT_RS05375 and BPHYT_RS05380 are separated by 152 nucleotides
BPHYT_RS05360: BPHYT_RS05360 - MFS transporter, at 1,233,097 to 1,234,416
_RS05360
BPHYT_RS05365: BPHYT_RS05365 - 3-ketoacyl-ACP reductase, at 1,234,572 to 1,235,330
_RS05365
BPHYT_RS05370: BPHYT_RS05370 - aldehyde dehydrogenase, at 1,235,374 to 1,236,813
_RS05370
BPHYT_RS05375: BPHYT_RS05375 - inorganic pyrophosphatase, at 1,237,067 to 1,237,594
_RS05375
BPHYT_RS05380: BPHYT_RS05380 - transcriptional regulator, at 1,237,747 to 1,238,085
_RS05380
Position (kb)
1235
1236
1237 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1234.448 kb on - strand at 1234.643 kb on - strand at 1234.949 kb on - strand, within BPHYT_RS05365 at 1235.315 kb on + strand at 1235.368 kb on - strand at 1235.417 kb on + strand at 1235.417 kb on + strand at 1235.502 kb on - strand at 1235.502 kb on - strand at 1235.502 kb on - strand at 1236.213 kb on + strand, within BPHYT_RS05370 at 1236.423 kb on - strand, within BPHYT_RS05370 at 1236.423 kb on - strand, within BPHYT_RS05370 at 1236.942 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,234,448 - -0.8 1,234,643 - -0.8 1,234,949 - BPHYT_RS05365 0.50 -0.1 1,235,315 + -1.0 1,235,368 - -0.6 1,235,417 + -0.8 1,235,417 + -0.8 1,235,502 - +0.3 1,235,502 - -1.8 1,235,502 - +0.8 1,236,213 + BPHYT_RS05370 0.58 -2.6 1,236,423 - BPHYT_RS05370 0.73 -3.2 1,236,423 - BPHYT_RS05370 0.73 -0.2 1,236,942 - -0.0
Or see this region's nucleotide sequence