Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05370

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05360 and BPHYT_RS05365 are separated by 155 nucleotidesBPHYT_RS05365 and BPHYT_RS05370 are separated by 43 nucleotidesBPHYT_RS05370 and BPHYT_RS05375 are separated by 253 nucleotidesBPHYT_RS05375 and BPHYT_RS05380 are separated by 152 nucleotides BPHYT_RS05360: BPHYT_RS05360 - MFS transporter, at 1,233,097 to 1,234,416 _RS05360 BPHYT_RS05365: BPHYT_RS05365 - 3-ketoacyl-ACP reductase, at 1,234,572 to 1,235,330 _RS05365 BPHYT_RS05370: BPHYT_RS05370 - aldehyde dehydrogenase, at 1,235,374 to 1,236,813 _RS05370 BPHYT_RS05375: BPHYT_RS05375 - inorganic pyrophosphatase, at 1,237,067 to 1,237,594 _RS05375 BPHYT_RS05380: BPHYT_RS05380 - transcriptional regulator, at 1,237,747 to 1,238,085 _RS05380 Position (kb) 1235 1236 1237Strain fitness (log2 ratio) -3 -2 -1 0 1at 1234.448 kb on - strandat 1234.643 kb on - strandat 1234.949 kb on - strand, within BPHYT_RS05365at 1235.315 kb on + strandat 1235.368 kb on - strandat 1235.417 kb on + strandat 1235.417 kb on + strandat 1235.502 kb on - strandat 1235.502 kb on - strandat 1235.502 kb on - strandat 1236.213 kb on + strand, within BPHYT_RS05370at 1236.423 kb on - strand, within BPHYT_RS05370at 1236.423 kb on - strand, within BPHYT_RS05370at 1236.942 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,234,448 - -0.8
1,234,643 - -0.8
1,234,949 - BPHYT_RS05365 0.50 -0.1
1,235,315 + -1.0
1,235,368 - -0.6
1,235,417 + -0.8
1,235,417 + -0.8
1,235,502 - +0.3
1,235,502 - -1.8
1,235,502 - +0.8
1,236,213 + BPHYT_RS05370 0.58 -2.6
1,236,423 - BPHYT_RS05370 0.73 -3.2
1,236,423 - BPHYT_RS05370 0.73 -0.2
1,236,942 - -0.0

Or see this region's nucleotide sequence