Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04215 and BPHYT_RS04220 are separated by 253 nucleotides BPHYT_RS04220 and BPHYT_RS04225 overlap by 4 nucleotides BPHYT_RS04225 and BPHYT_RS04230 overlap by 14 nucleotides
BPHYT_RS04215: BPHYT_RS04215 - hypothetical protein, at 966,322 to 967,734
_RS04215
BPHYT_RS04220: BPHYT_RS04220 - hypothetical protein, at 967,988 to 968,566
_RS04220
BPHYT_RS04225: BPHYT_RS04225 - Holliday junction resolvase, at 968,563 to 969,003
_RS04225
BPHYT_RS04230: BPHYT_RS04230 - uracil phosphoribosyltransferase, at 968,990 to 969,529
_RS04230
Position (kb) 967
968
969 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 967.190 kb on - strand, within BPHYT_RS04215 at 967.336 kb on + strand, within BPHYT_RS04215 at 967.336 kb on + strand, within BPHYT_RS04215 at 967.820 kb on + strand at 967.820 kb on + strand at 967.820 kb on + strand at 967.820 kb on + strand at 967.820 kb on + strand at 967.821 kb on - strand at 967.821 kb on - strand at 967.821 kb on - strand at 967.876 kb on + strand at 967.877 kb on - strand at 967.888 kb on + strand at 967.888 kb on + strand at 967.987 kb on + strand at 967.987 kb on + strand at 967.987 kb on + strand at 967.987 kb on + strand at 967.988 kb on - strand at 968.081 kb on + strand, within BPHYT_RS04220 at 968.213 kb on + strand, within BPHYT_RS04220 at 968.213 kb on + strand, within BPHYT_RS04220 at 968.279 kb on + strand, within BPHYT_RS04220 at 968.969 kb on + strand at 969.051 kb on - strand, within BPHYT_RS04230 at 969.203 kb on + strand, within BPHYT_RS04230 at 969.203 kb on + strand, within BPHYT_RS04230 at 969.204 kb on - strand, within BPHYT_RS04230 at 969.296 kb on + strand, within BPHYT_RS04230 at 969.296 kb on + strand, within BPHYT_RS04230 at 969.297 kb on - strand, within BPHYT_RS04230 at 969.335 kb on + strand, within BPHYT_RS04230 at 969.341 kb on + strand, within BPHYT_RS04230 at 969.342 kb on - strand, within BPHYT_RS04230
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 967,190 - BPHYT_RS04215 0.61 -1.5 967,336 + BPHYT_RS04215 0.72 -0.8 967,336 + BPHYT_RS04215 0.72 +0.6 967,820 + -0.7 967,820 + -2.1 967,820 + +0.6 967,820 + -0.3 967,820 + +0.7 967,821 - +0.1 967,821 - -0.2 967,821 - +0.0 967,876 + -0.9 967,877 - +1.6 967,888 + +0.1 967,888 + -0.6 967,987 + +0.1 967,987 + +0.6 967,987 + +0.2 967,987 + +0.0 967,988 - +2.0 968,081 + BPHYT_RS04220 0.16 -0.4 968,213 + BPHYT_RS04220 0.39 -1.2 968,213 + BPHYT_RS04220 0.39 -3.6 968,279 + BPHYT_RS04220 0.50 -0.8 968,969 + +0.1 969,051 - BPHYT_RS04230 0.11 +1.2 969,203 + BPHYT_RS04230 0.39 -2.5 969,203 + BPHYT_RS04230 0.39 -0.2 969,204 - BPHYT_RS04230 0.40 -1.1 969,296 + BPHYT_RS04230 0.57 -0.9 969,296 + BPHYT_RS04230 0.57 +1.0 969,297 - BPHYT_RS04230 0.57 +0.8 969,335 + BPHYT_RS04230 0.64 +1.2 969,341 + BPHYT_RS04230 0.65 +0.1 969,342 - BPHYT_RS04230 0.65 -1.1
Or see this region's nucleotide sequence