Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04140 and BPHYT_RS04145 are separated by 117 nucleotides BPHYT_RS04145 and BPHYT_RS04150 overlap by 4 nucleotides
BPHYT_RS04140: BPHYT_RS04140 - polysaccharide biosynthesis protein, at 948,609 to 949,868
_RS04140
BPHYT_RS04145: BPHYT_RS04145 - family 2 glycosyl transferase, at 949,986 to 950,957
_RS04145
BPHYT_RS04150: BPHYT_RS04150 - UDP-galactopyranose mutase, at 950,954 to 952,045
_RS04150
Position (kb) 949
950
951 Strain fitness (log2 ratio) -2
-1
0
1
2 at 949.174 kb on + strand, within BPHYT_RS04140 at 949.287 kb on + strand, within BPHYT_RS04140 at 949.682 kb on + strand, within BPHYT_RS04140 at 949.946 kb on + strand at 949.947 kb on - strand at 950.026 kb on - strand at 950.182 kb on - strand, within BPHYT_RS04145 at 950.228 kb on + strand, within BPHYT_RS04145 at 950.228 kb on + strand, within BPHYT_RS04145 at 950.310 kb on - strand, within BPHYT_RS04145 at 950.310 kb on - strand, within BPHYT_RS04145 at 950.372 kb on - strand, within BPHYT_RS04145 at 950.465 kb on + strand, within BPHYT_RS04145 at 950.466 kb on - strand, within BPHYT_RS04145 at 950.687 kb on - strand, within BPHYT_RS04145 at 950.832 kb on - strand, within BPHYT_RS04145 at 950.838 kb on - strand, within BPHYT_RS04145 at 951.013 kb on - strand at 951.121 kb on - strand, within BPHYT_RS04150 at 951.217 kb on - strand, within BPHYT_RS04150 at 951.357 kb on + strand, within BPHYT_RS04150 at 951.694 kb on - strand, within BPHYT_RS04150 at 951.838 kb on - strand, within BPHYT_RS04150
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 949,174 + BPHYT_RS04140 0.45 +0.8 949,287 + BPHYT_RS04140 0.54 +0.6 949,682 + BPHYT_RS04140 0.85 -0.1 949,946 + -1.4 949,947 - -0.2 950,026 - +0.4 950,182 - BPHYT_RS04145 0.20 -0.0 950,228 + BPHYT_RS04145 0.25 -0.9 950,228 + BPHYT_RS04145 0.25 -1.3 950,310 - BPHYT_RS04145 0.33 -0.6 950,310 - BPHYT_RS04145 0.33 -0.6 950,372 - BPHYT_RS04145 0.40 -1.8 950,465 + BPHYT_RS04145 0.49 -0.2 950,466 - BPHYT_RS04145 0.49 +0.3 950,687 - BPHYT_RS04145 0.72 -1.9 950,832 - BPHYT_RS04145 0.87 +0.6 950,838 - BPHYT_RS04145 0.88 +0.7 951,013 - +1.2 951,121 - BPHYT_RS04150 0.15 -0.2 951,217 - BPHYT_RS04150 0.24 -2.0 951,357 + BPHYT_RS04150 0.37 +2.2 951,694 - BPHYT_RS04150 0.68 -0.1 951,838 - BPHYT_RS04150 0.81 -0.4
Or see this region's nucleotide sequence