Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02995 and BPHYT_RS03000 are separated by 10 nucleotides BPHYT_RS03000 and BPHYT_RS03005 overlap by 4 nucleotides
BPHYT_RS02995: BPHYT_RS02995 - 2-methylcitrate dehydratase, at 667,181 to 668,593
_RS02995
BPHYT_RS03000: BPHYT_RS03000 - acyl-CoA dehydrogenase, at 668,604 to 669,767
_RS03000
BPHYT_RS03005: BPHYT_RS03005 - CoA transferase, at 669,764 to 670,954
_RS03005
Position (kb) 668
669
670 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 668.210 kb on + strand, within BPHYT_RS02995 at 668.211 kb on - strand, within BPHYT_RS02995 at 668.211 kb on - strand, within BPHYT_RS02995 at 668.352 kb on - strand, within BPHYT_RS02995 at 668.468 kb on + strand at 668.526 kb on + strand at 668.527 kb on - strand at 668.591 kb on + strand at 668.592 kb on - strand at 668.717 kb on + strand at 668.817 kb on + strand, within BPHYT_RS03000 at 668.817 kb on + strand, within BPHYT_RS03000 at 668.933 kb on + strand, within BPHYT_RS03000 at 668.934 kb on - strand, within BPHYT_RS03000 at 668.937 kb on + strand, within BPHYT_RS03000 at 668.938 kb on - strand, within BPHYT_RS03000 at 669.052 kb on - strand, within BPHYT_RS03000 at 669.224 kb on + strand, within BPHYT_RS03000 at 669.225 kb on - strand, within BPHYT_RS03000 at 669.319 kb on + strand, within BPHYT_RS03000 at 669.406 kb on - strand, within BPHYT_RS03000 at 669.406 kb on - strand, within BPHYT_RS03000 at 669.406 kb on - strand, within BPHYT_RS03000 at 669.532 kb on - strand, within BPHYT_RS03000 at 669.532 kb on - strand, within BPHYT_RS03000 at 669.637 kb on - strand, within BPHYT_RS03000 at 669.654 kb on + strand at 669.655 kb on - strand at 669.693 kb on - strand at 669.693 kb on - strand at 670.095 kb on - strand, within BPHYT_RS03005 at 670.095 kb on - strand, within BPHYT_RS03005 at 670.148 kb on + strand, within BPHYT_RS03005 at 670.190 kb on + strand, within BPHYT_RS03005 at 670.191 kb on - strand, within BPHYT_RS03005 at 670.387 kb on + strand, within BPHYT_RS03005 at 670.388 kb on - strand, within BPHYT_RS03005 at 670.398 kb on - strand, within BPHYT_RS03005 at 670.413 kb on - strand, within BPHYT_RS03005 at 670.484 kb on + strand, within BPHYT_RS03005 at 670.484 kb on + strand, within BPHYT_RS03005 at 670.484 kb on + strand, within BPHYT_RS03005
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 668,210 + BPHYT_RS02995 0.73 +0.2 668,211 - BPHYT_RS02995 0.73 -1.3 668,211 - BPHYT_RS02995 0.73 -0.8 668,352 - BPHYT_RS02995 0.83 -1.0 668,468 + +0.9 668,526 + -1.6 668,527 - -0.4 668,591 + +0.2 668,592 - +0.4 668,717 + +1.2 668,817 + BPHYT_RS03000 0.18 +0.2 668,817 + BPHYT_RS03000 0.18 -0.4 668,933 + BPHYT_RS03000 0.28 -0.6 668,934 - BPHYT_RS03000 0.28 -0.4 668,937 + BPHYT_RS03000 0.29 -1.9 668,938 - BPHYT_RS03000 0.29 -0.7 669,052 - BPHYT_RS03000 0.38 -2.0 669,224 + BPHYT_RS03000 0.53 -0.5 669,225 - BPHYT_RS03000 0.53 -2.6 669,319 + BPHYT_RS03000 0.61 -0.1 669,406 - BPHYT_RS03000 0.69 +1.2 669,406 - BPHYT_RS03000 0.69 -0.1 669,406 - BPHYT_RS03000 0.69 -0.9 669,532 - BPHYT_RS03000 0.80 +1.0 669,532 - BPHYT_RS03000 0.80 +0.3 669,637 - BPHYT_RS03000 0.89 +0.8 669,654 + +1.3 669,655 - +3.2 669,693 - +0.9 669,693 - +0.0 670,095 - BPHYT_RS03005 0.28 +1.6 670,095 - BPHYT_RS03005 0.28 -0.9 670,148 + BPHYT_RS03005 0.32 -1.5 670,190 + BPHYT_RS03005 0.36 -0.3 670,191 - BPHYT_RS03005 0.36 -0.3 670,387 + BPHYT_RS03005 0.52 -1.4 670,388 - BPHYT_RS03005 0.52 +0.3 670,398 - BPHYT_RS03005 0.53 -0.9 670,413 - BPHYT_RS03005 0.54 +1.8 670,484 + BPHYT_RS03005 0.60 -0.6 670,484 + BPHYT_RS03005 0.60 +0.3 670,484 + BPHYT_RS03005 0.60 +0.1
Or see this region's nucleotide sequence