Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02970 and BPHYT_RS02975 are separated by 386 nucleotides BPHYT_RS02975 and BPHYT_RS02980 are separated by 135 nucleotides BPHYT_RS02980 and BPHYT_RS02985 are separated by 296 nucleotides
BPHYT_RS02970: BPHYT_RS02970 - excinuclease ABC subunit A, at 659,698 to 662,571
_RS02970
BPHYT_RS02975: BPHYT_RS02975 - membrane protein, at 662,958 to 664,115
_RS02975
BPHYT_RS02980: BPHYT_RS02980 - single-stranded DNA-binding protein, at 664,251 to 664,790
_RS02980
BPHYT_RS02985: BPHYT_RS02985 - MaoC family dehydratase, at 665,087 to 665,587
_RS02985
Position (kb) 662
663
664
665 Strain fitness (log2 ratio) -1
0
1
2 at 661.991 kb on + strand, within BPHYT_RS02970 at 661.991 kb on + strand, within BPHYT_RS02970 at 661.992 kb on - strand, within BPHYT_RS02970 at 661.992 kb on - strand, within BPHYT_RS02970 at 661.992 kb on - strand, within BPHYT_RS02970 at 661.992 kb on - strand, within BPHYT_RS02970 at 662.217 kb on - strand, within BPHYT_RS02970 at 662.252 kb on + strand, within BPHYT_RS02970 at 662.252 kb on + strand, within BPHYT_RS02970 at 662.253 kb on - strand, within BPHYT_RS02970 at 662.253 kb on - strand, within BPHYT_RS02970 at 662.394 kb on - strand at 662.414 kb on + strand at 662.415 kb on - strand at 662.415 kb on - strand at 662.510 kb on - strand at 662.610 kb on - strand at 662.618 kb on + strand at 662.618 kb on + strand at 662.618 kb on + strand at 662.618 kb on + strand at 662.621 kb on + strand at 662.621 kb on + strand at 662.621 kb on + strand at 662.621 kb on + strand at 662.621 kb on + strand at 662.687 kb on + strand at 662.745 kb on - strand at 663.147 kb on + strand, within BPHYT_RS02975 at 663.619 kb on + strand, within BPHYT_RS02975 at 663.620 kb on - strand, within BPHYT_RS02975 at 663.684 kb on + strand, within BPHYT_RS02975 at 663.954 kb on + strand, within BPHYT_RS02975 at 664.074 kb on - strand at 664.074 kb on - strand at 664.075 kb on + strand at 664.947 kb on + strand at 665.019 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 661,991 + BPHYT_RS02970 0.80 +0.3 661,991 + BPHYT_RS02970 0.80 -1.5 661,992 - BPHYT_RS02970 0.80 -0.2 661,992 - BPHYT_RS02970 0.80 -0.6 661,992 - BPHYT_RS02970 0.80 -0.5 661,992 - BPHYT_RS02970 0.80 +0.3 662,217 - BPHYT_RS02970 0.88 +0.3 662,252 + BPHYT_RS02970 0.89 -0.1 662,252 + BPHYT_RS02970 0.89 +1.0 662,253 - BPHYT_RS02970 0.89 -1.6 662,253 - BPHYT_RS02970 0.89 -0.9 662,394 - +0.4 662,414 + -0.4 662,415 - -0.2 662,415 - +0.3 662,510 - +1.2 662,610 - +0.2 662,618 + +0.2 662,618 + -0.2 662,618 + +0.9 662,618 + +0.5 662,621 + +0.6 662,621 + -0.5 662,621 + -0.3 662,621 + +2.0 662,621 + -0.4 662,687 + -1.3 662,745 - +2.6 663,147 + BPHYT_RS02975 0.16 +2.0 663,619 + BPHYT_RS02975 0.57 +2.6 663,620 - BPHYT_RS02975 0.57 +2.6 663,684 + BPHYT_RS02975 0.63 -0.0 663,954 + BPHYT_RS02975 0.86 +2.0 664,074 - +1.6 664,074 - +2.6 664,075 + -0.0 664,947 + +2.6 665,019 + -1.2
Or see this region's nucleotide sequence