Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02740 and BPHYT_RS02745 are separated by 109 nucleotides BPHYT_RS02745 and BPHYT_RS02750 are separated by 255 nucleotides
BPHYT_RS02740: BPHYT_RS02740 - N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (from data) , at 613,621 to 616,185
_RS02740
BPHYT_RS02745: BPHYT_RS02745 - N-acetylglucosamine-specific PTS system, IIBC components (nagE) (from data) , at 616,295 to 618,070
_RS02745
BPHYT_RS02750: BPHYT_RS02750 - hypothetical protein, at 618,326 to 619,651
_RS02750
Position (kb)
616
617
618
619 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 615.430 kb on + strand, within BPHYT_RS02740 at 615.431 kb on - strand, within BPHYT_RS02740 at 615.431 kb on - strand, within BPHYT_RS02740 at 615.431 kb on - strand, within BPHYT_RS02740 at 615.691 kb on - strand, within BPHYT_RS02740 at 616.201 kb on + strand at 616.228 kb on + strand at 616.228 kb on + strand at 616.228 kb on + strand at 616.232 kb on + strand at 616.232 kb on + strand at 616.643 kb on + strand, within BPHYT_RS02745 at 616.752 kb on + strand, within BPHYT_RS02745 at 616.753 kb on - strand, within BPHYT_RS02745 at 617.174 kb on + strand, within BPHYT_RS02745 at 617.174 kb on + strand, within BPHYT_RS02745 at 617.200 kb on + strand, within BPHYT_RS02745 at 617.270 kb on + strand, within BPHYT_RS02745 at 617.282 kb on + strand, within BPHYT_RS02745 at 617.283 kb on - strand, within BPHYT_RS02745 at 617.283 kb on - strand, within BPHYT_RS02745 at 617.330 kb on + strand, within BPHYT_RS02745 at 617.331 kb on - strand, within BPHYT_RS02745 at 617.332 kb on + strand, within BPHYT_RS02745 at 617.333 kb on - strand, within BPHYT_RS02745 at 617.345 kb on + strand, within BPHYT_RS02745 at 617.494 kb on + strand, within BPHYT_RS02745 at 617.896 kb on + strand at 617.981 kb on - strand at 618.069 kb on - strand at 618.421 kb on + strand at 618.421 kb on + strand at 618.509 kb on + strand, within BPHYT_RS02750 at 618.510 kb on - strand, within BPHYT_RS02750 at 618.514 kb on + strand, within BPHYT_RS02750 at 618.515 kb on - strand, within BPHYT_RS02750 at 618.515 kb on - strand, within BPHYT_RS02750 at 618.595 kb on - strand, within BPHYT_RS02750 at 618.638 kb on + strand at 618.638 kb on + strand, within BPHYT_RS02750 at 618.639 kb on - strand, within BPHYT_RS02750 at 618.641 kb on + strand, within BPHYT_RS02750 at 618.642 kb on - strand, within BPHYT_RS02750 at 618.642 kb on - strand, within BPHYT_RS02750 at 618.744 kb on - strand, within BPHYT_RS02750 at 618.766 kb on + strand, within BPHYT_RS02750 at 618.818 kb on + strand, within BPHYT_RS02750 at 618.885 kb on + strand, within BPHYT_RS02750 at 618.904 kb on + strand, within BPHYT_RS02750 at 618.983 kb on + strand, within BPHYT_RS02750 at 618.983 kb on + strand, within BPHYT_RS02750
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 615,430 + BPHYT_RS02740 0.71 +0.1 615,431 - BPHYT_RS02740 0.71 +0.5 615,431 - BPHYT_RS02740 0.71 +0.3 615,431 - BPHYT_RS02740 0.71 -2.5 615,691 - BPHYT_RS02740 0.81 +0.5 616,201 + +0.7 616,228 + +0.0 616,228 + +0.8 616,228 + +0.2 616,232 + -0.9 616,232 + -0.1 616,643 + BPHYT_RS02745 0.20 -1.3 616,752 + BPHYT_RS02745 0.26 -0.7 616,753 - BPHYT_RS02745 0.26 +0.1 617,174 + BPHYT_RS02745 0.49 -0.4 617,174 + BPHYT_RS02745 0.49 -1.6 617,200 + BPHYT_RS02745 0.51 +1.2 617,270 + BPHYT_RS02745 0.55 +0.0 617,282 + BPHYT_RS02745 0.56 -1.9 617,283 - BPHYT_RS02745 0.56 -0.6 617,283 - BPHYT_RS02745 0.56 -0.5 617,330 + BPHYT_RS02745 0.58 -0.3 617,331 - BPHYT_RS02745 0.58 -1.4 617,332 + BPHYT_RS02745 0.58 -1.2 617,333 - BPHYT_RS02745 0.58 -1.1 617,345 + BPHYT_RS02745 0.59 -0.8 617,494 + BPHYT_RS02745 0.68 -0.5 617,896 + +0.7 617,981 - -0.4 618,069 - +0.1 618,421 + -0.6 618,421 + +0.4 618,509 + BPHYT_RS02750 0.14 +0.1 618,510 - BPHYT_RS02750 0.14 -0.1 618,514 + BPHYT_RS02750 0.14 +0.5 618,515 - BPHYT_RS02750 0.14 +0.1 618,515 - BPHYT_RS02750 0.14 -1.3 618,595 - BPHYT_RS02750 0.20 -0.5 618,638 + +2.6 618,638 + BPHYT_RS02750 0.24 +0.6 618,639 - BPHYT_RS02750 0.24 +0.0 618,641 + BPHYT_RS02750 0.24 -1.1 618,642 - BPHYT_RS02750 0.24 +0.1 618,642 - BPHYT_RS02750 0.24 -0.7 618,744 - BPHYT_RS02750 0.32 -0.5 618,766 + BPHYT_RS02750 0.33 -0.2 618,818 + BPHYT_RS02750 0.37 -0.5 618,885 + BPHYT_RS02750 0.42 -2.6 618,904 + BPHYT_RS02750 0.44 +0.6 618,983 + BPHYT_RS02750 0.50 -0.1 618,983 + BPHYT_RS02750 0.50 +1.2
Or see this region's nucleotide sequence