Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02560 and BPHYT_RS02565 are separated by 337 nucleotides BPHYT_RS02565 and BPHYT_RS02570 are separated by 96 nucleotides BPHYT_RS02570 and BPHYT_RS02575 are separated by 305 nucleotides
BPHYT_RS02560: BPHYT_RS02560 - glutathione synthetase, at 581,572 to 582,528
_RS02560
BPHYT_RS02565: BPHYT_RS02565 - PTS mannose transporter subunit IIA, at 582,866 to 583,393
_RS02565
BPHYT_RS02570: BPHYT_RS02570 - phosphocarrier protein HPr, at 583,490 to 583,759
_RS02570
BPHYT_RS02575: BPHYT_RS02575 - phosphoenolpyruvate-protein phosphotransferase, at 584,065 to 585,813
_RS02575
Position (kb) 583
584 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 582.584 kb on + strand at 582.584 kb on + strand at 582.585 kb on - strand at 582.633 kb on + strand at 582.634 kb on - strand at 582.634 kb on - strand at 582.634 kb on - strand at 582.634 kb on - strand at 582.701 kb on + strand at 583.389 kb on + strand at 583.391 kb on + strand at 583.391 kb on + strand at 583.391 kb on + strand at 583.392 kb on - strand at 583.392 kb on - strand at 583.449 kb on + strand at 583.468 kb on + strand at 583.511 kb on - strand at 583.511 kb on - strand at 583.511 kb on - strand at 583.574 kb on + strand, within BPHYT_RS02570 at 583.575 kb on - strand, within BPHYT_RS02570 at 583.575 kb on - strand, within BPHYT_RS02570 at 583.994 kb on + strand at 583.995 kb on - strand at 584.051 kb on + strand at 584.052 kb on - strand at 584.052 kb on - strand at 584.150 kb on + strand at 584.158 kb on + strand at 584.158 kb on + strand at 584.159 kb on - strand at 584.227 kb on + strand at 584.437 kb on + strand, within BPHYT_RS02575 at 584.736 kb on + strand, within BPHYT_RS02575
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 582,584 + +0.6 582,584 + -0.4 582,585 - +0.5 582,633 + +0.8 582,634 - -0.6 582,634 - +1.6 582,634 - +0.2 582,634 - +0.9 582,701 + -0.4 583,389 + +4.6 583,391 + -0.4 583,391 + +1.0 583,391 + +1.6 583,392 - +0.1 583,392 - +0.5 583,449 + -0.5 583,468 + +1.8 583,511 - -1.4 583,511 - -1.9 583,511 - -1.9 583,574 + BPHYT_RS02570 0.31 -1.9 583,575 - BPHYT_RS02570 0.31 -1.6 583,575 - BPHYT_RS02570 0.31 -1.7 583,994 + -0.7 583,995 - +0.2 584,051 + +1.0 584,052 - -2.0 584,052 - -1.2 584,150 + -4.5 584,158 + -1.6 584,158 + -1.9 584,159 - -1.6 584,227 + -1.3 584,437 + BPHYT_RS02575 0.21 -1.8 584,736 + BPHYT_RS02575 0.38 -1.5
Or see this region's nucleotide sequence