Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01295 and BPHYT_RS01300 are separated by 87 nucleotides BPHYT_RS01300 and BPHYT_RS01305 are separated by 116 nucleotides BPHYT_RS01305 and BPHYT_RS01310 are separated by 115 nucleotides
BPHYT_RS01295: BPHYT_RS01295 - glutathione S-transferase, at 296,422 to 297,096
_RS01295
BPHYT_RS01300: BPHYT_RS01300 - ArsR family transcriptional regulator, at 297,184 to 297,879
_RS01300
BPHYT_RS01305: BPHYT_RS01305 - MFS transporter, at 297,996 to 299,387
_RS01305
BPHYT_RS01310: BPHYT_RS01310 - L-lysine 6-monooxygenase, at 299,503 to 300,828
_RS01310
Position (kb) 297
298
299
300 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 297.059 kb on + strand at 297.094 kb on + strand at 297.193 kb on - strand at 297.196 kb on - strand at 297.339 kb on - strand, within BPHYT_RS01300 at 297.542 kb on + strand, within BPHYT_RS01300 at 297.672 kb on - strand, within BPHYT_RS01300 at 297.672 kb on - strand, within BPHYT_RS01300 at 297.811 kb on - strand at 297.864 kb on + strand at 297.864 kb on + strand at 297.864 kb on + strand at 297.865 kb on - strand at 297.897 kb on + strand at 297.907 kb on + strand at 297.976 kb on - strand at 297.993 kb on + strand at 298.045 kb on + strand at 298.082 kb on - strand at 298.085 kb on + strand at 298.085 kb on + strand at 298.085 kb on + strand at 298.086 kb on - strand at 298.086 kb on - strand at 298.139 kb on + strand, within BPHYT_RS01305 at 298.171 kb on - strand, within BPHYT_RS01305 at 298.173 kb on - strand, within BPHYT_RS01305 at 298.173 kb on - strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.538 kb on - strand, within BPHYT_RS01305 at 298.875 kb on + strand, within BPHYT_RS01305 at 298.986 kb on + strand, within BPHYT_RS01305 at 299.025 kb on + strand, within BPHYT_RS01305 at 299.073 kb on + strand, within BPHYT_RS01305 at 299.126 kb on + strand, within BPHYT_RS01305 at 299.336 kb on - strand at 299.353 kb on + strand at 299.497 kb on + strand at 299.498 kb on - strand at 299.511 kb on + strand at 299.511 kb on + strand at 299.549 kb on + strand at 299.683 kb on + strand, within BPHYT_RS01310 at 299.719 kb on - strand, within BPHYT_RS01310 at 299.719 kb on - strand, within BPHYT_RS01310 at 299.796 kb on - strand, within BPHYT_RS01310 at 299.818 kb on + strand, within BPHYT_RS01310 at 299.818 kb on + strand, within BPHYT_RS01310 at 299.819 kb on - strand, within BPHYT_RS01310 at 300.011 kb on + strand, within BPHYT_RS01310 at 300.025 kb on + strand, within BPHYT_RS01310 at 300.026 kb on - strand, within BPHYT_RS01310 at 300.209 kb on - strand, within BPHYT_RS01310 at 300.209 kb on - strand, within BPHYT_RS01310 at 300.211 kb on - strand, within BPHYT_RS01310 at 300.211 kb on - strand, within BPHYT_RS01310 at 300.211 kb on - strand, within BPHYT_RS01310 at 300.221 kb on - strand, within BPHYT_RS01310 at 300.221 kb on - strand, within BPHYT_RS01310 at 300.229 kb on + strand, within BPHYT_RS01310 at 300.230 kb on - strand, within BPHYT_RS01310 at 300.230 kb on - strand, within BPHYT_RS01310 at 300.321 kb on + strand, within BPHYT_RS01310 at 300.325 kb on + strand, within BPHYT_RS01310
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 297,059 + -0.5 297,094 + +4.7 297,193 - +1.0 297,196 - +0.4 297,339 - BPHYT_RS01300 0.22 -0.5 297,542 + BPHYT_RS01300 0.51 -2.3 297,672 - BPHYT_RS01300 0.70 -1.5 297,672 - BPHYT_RS01300 0.70 +1.1 297,811 - -1.3 297,864 + -0.3 297,864 + +1.7 297,864 + +0.5 297,865 - +0.4 297,897 + +0.1 297,907 + +2.7 297,976 - +1.1 297,993 + +1.1 298,045 + +0.4 298,082 - +0.4 298,085 + -1.5 298,085 + -0.4 298,085 + -0.8 298,086 - +0.4 298,086 - -0.6 298,139 + BPHYT_RS01305 0.10 +0.7 298,171 - BPHYT_RS01305 0.13 +1.1 298,173 - BPHYT_RS01305 0.13 -0.8 298,173 - BPHYT_RS01305 0.13 +0.4 298,382 + BPHYT_RS01305 0.28 +0.7 298,382 + BPHYT_RS01305 0.28 +0.8 298,382 + BPHYT_RS01305 0.28 -0.6 298,382 + BPHYT_RS01305 0.28 -0.6 298,538 - BPHYT_RS01305 0.39 +0.4 298,875 + BPHYT_RS01305 0.63 +0.2 298,986 + BPHYT_RS01305 0.71 +1.0 299,025 + BPHYT_RS01305 0.74 +0.6 299,073 + BPHYT_RS01305 0.77 +0.0 299,126 + BPHYT_RS01305 0.81 +0.1 299,336 - +1.1 299,353 + -1.2 299,497 + +2.4 299,498 - -1.8 299,511 + +0.6 299,511 + +0.1 299,549 + +0.3 299,683 + BPHYT_RS01310 0.14 -1.1 299,719 - BPHYT_RS01310 0.16 -0.0 299,719 - BPHYT_RS01310 0.16 +0.2 299,796 - BPHYT_RS01310 0.22 -0.3 299,818 + BPHYT_RS01310 0.24 +0.1 299,818 + BPHYT_RS01310 0.24 +0.4 299,819 - BPHYT_RS01310 0.24 -0.7 300,011 + BPHYT_RS01310 0.38 +1.2 300,025 + BPHYT_RS01310 0.39 -0.5 300,026 - BPHYT_RS01310 0.39 +0.6 300,209 - BPHYT_RS01310 0.53 +1.7 300,209 - BPHYT_RS01310 0.53 -0.7 300,211 - BPHYT_RS01310 0.53 -0.0 300,211 - BPHYT_RS01310 0.53 -0.9 300,211 - BPHYT_RS01310 0.53 -0.4 300,221 - BPHYT_RS01310 0.54 -0.6 300,221 - BPHYT_RS01310 0.54 +0.9 300,229 + BPHYT_RS01310 0.55 -1.2 300,230 - BPHYT_RS01310 0.55 -0.7 300,230 - BPHYT_RS01310 0.55 -0.4 300,321 + BPHYT_RS01310 0.62 +1.7 300,325 + BPHYT_RS01310 0.62 -1.7
Or see this region's nucleotide sequence