Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00810 and BPHYT_RS00815 are separated by 14 nucleotides BPHYT_RS00815 and BPHYT_RS00820 overlap by 4 nucleotides BPHYT_RS00820 and BPHYT_RS00825 overlap by 4 nucleotides
BPHYT_RS00810: BPHYT_RS00810 - type IV secretion system protein VirB11, at 183,124 to 184,251
_RS00810
BPHYT_RS00815: BPHYT_RS00815 - hypothetical protein, at 184,266 to 184,676
_RS00815
BPHYT_RS00820: BPHYT_RS00820 - type IV secretory pathway protein VirD4, at 184,673 to 186,457
_RS00820
BPHYT_RS00825: BPHYT_RS00825 - DNA replication protein, at 186,454 to 188,367
_RS00825
Position (kb) 184
185
186
187 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 183.697 kb on - strand, within BPHYT_RS00810 at 183.714 kb on + strand, within BPHYT_RS00810 at 183.844 kb on + strand, within BPHYT_RS00810 at 183.845 kb on - strand, within BPHYT_RS00810 at 183.845 kb on - strand, within BPHYT_RS00810 at 183.959 kb on - strand, within BPHYT_RS00810 at 184.009 kb on + strand, within BPHYT_RS00810 at 184.010 kb on - strand, within BPHYT_RS00810 at 184.140 kb on + strand at 184.141 kb on - strand at 184.141 kb on - strand at 184.584 kb on + strand, within BPHYT_RS00815 at 184.745 kb on + strand at 184.813 kb on + strand at 184.957 kb on + strand, within BPHYT_RS00820 at 184.958 kb on - strand, within BPHYT_RS00820 at 184.958 kb on - strand, within BPHYT_RS00820 at 184.967 kb on + strand, within BPHYT_RS00820 at 185.225 kb on + strand, within BPHYT_RS00820 at 185.226 kb on - strand, within BPHYT_RS00820 at 185.630 kb on + strand, within BPHYT_RS00820 at 185.630 kb on + strand, within BPHYT_RS00820 at 185.642 kb on + strand, within BPHYT_RS00820 at 185.642 kb on + strand, within BPHYT_RS00820 at 185.697 kb on - strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.750 kb on + strand, within BPHYT_RS00820 at 185.751 kb on - strand, within BPHYT_RS00820 at 185.751 kb on - strand, within BPHYT_RS00820 at 185.751 kb on - strand, within BPHYT_RS00820 at 185.783 kb on + strand, within BPHYT_RS00820 at 185.784 kb on - strand, within BPHYT_RS00820 at 186.227 kb on + strand, within BPHYT_RS00820 at 186.466 kb on + strand at 186.619 kb on + strand at 186.619 kb on + strand at 186.619 kb on + strand at 186.619 kb on + strand at 186.907 kb on + strand, within BPHYT_RS00825 at 186.908 kb on - strand, within BPHYT_RS00825 at 187.457 kb on + strand, within BPHYT_RS00825
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 183,697 - BPHYT_RS00810 0.51 +1.1 183,714 + BPHYT_RS00810 0.52 +0.0 183,844 + BPHYT_RS00810 0.64 +0.4 183,845 - BPHYT_RS00810 0.64 -0.2 183,845 - BPHYT_RS00810 0.64 -0.5 183,959 - BPHYT_RS00810 0.74 -0.3 184,009 + BPHYT_RS00810 0.78 +0.4 184,010 - BPHYT_RS00810 0.79 -1.0 184,140 + -1.0 184,141 - +0.1 184,141 - +0.8 184,584 + BPHYT_RS00815 0.77 -0.7 184,745 + -1.5 184,813 + -0.3 184,957 + BPHYT_RS00820 0.16 +1.0 184,958 - BPHYT_RS00820 0.16 -0.5 184,958 - BPHYT_RS00820 0.16 +0.8 184,967 + BPHYT_RS00820 0.16 +0.8 185,225 + BPHYT_RS00820 0.31 -3.0 185,226 - BPHYT_RS00820 0.31 -0.6 185,630 + BPHYT_RS00820 0.54 -0.3 185,630 + BPHYT_RS00820 0.54 -1.4 185,642 + BPHYT_RS00820 0.54 -0.5 185,642 + BPHYT_RS00820 0.54 +0.1 185,697 - BPHYT_RS00820 0.57 -0.3 185,750 + BPHYT_RS00820 0.60 +2.1 185,750 + BPHYT_RS00820 0.60 +0.1 185,750 + BPHYT_RS00820 0.60 -0.5 185,750 + BPHYT_RS00820 0.60 -0.1 185,751 - BPHYT_RS00820 0.60 -0.8 185,751 - BPHYT_RS00820 0.60 +2.0 185,751 - BPHYT_RS00820 0.60 +0.1 185,783 + BPHYT_RS00820 0.62 -0.4 185,784 - BPHYT_RS00820 0.62 +0.2 186,227 + BPHYT_RS00820 0.87 -0.6 186,466 + -0.9 186,619 + -0.0 186,619 + +0.2 186,619 + +0.2 186,619 + +0.2 186,907 + BPHYT_RS00825 0.24 -0.1 186,908 - BPHYT_RS00825 0.24 +0.9 187,457 + BPHYT_RS00825 0.52 +1.1
Or see this region's nucleotide sequence