Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00535 and BPHYT_RS00540 are separated by 389 nucleotides BPHYT_RS00540 and BPHYT_RS00545 are separated by 434 nucleotides
BPHYT_RS00535: BPHYT_RS00535 - AraC family transcriptional regulator, at 129,281 to 130,255
_RS00535
BPHYT_RS00540: BPHYT_RS00540 - hypothetical protein, at 130,645 to 131,079
_RS00540
BPHYT_RS00545: BPHYT_RS00545 - LysR family transcriptional regulator, at 131,514 to 132,455
_RS00545
Position (kb) 130
131
132 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 129.751 kb on + strand, within BPHYT_RS00535 at 129.752 kb on - strand, within BPHYT_RS00535 at 129.771 kb on - strand, within BPHYT_RS00535 at 129.771 kb on - strand, within BPHYT_RS00535 at 129.899 kb on + strand, within BPHYT_RS00535 at 129.900 kb on - strand, within BPHYT_RS00535 at 130.111 kb on + strand, within BPHYT_RS00535 at 130.111 kb on + strand, within BPHYT_RS00535 at 130.135 kb on + strand, within BPHYT_RS00535 at 130.136 kb on - strand, within BPHYT_RS00535 at 130.188 kb on + strand at 130.189 kb on - strand at 130.311 kb on - strand at 130.432 kb on - strand at 130.514 kb on + strand at 130.515 kb on - strand at 130.515 kb on - strand at 130.586 kb on - strand at 130.916 kb on - strand, within BPHYT_RS00540 at 130.951 kb on + strand, within BPHYT_RS00540 at 130.969 kb on + strand, within BPHYT_RS00540 at 130.979 kb on - strand, within BPHYT_RS00540 at 131.152 kb on + strand at 131.280 kb on + strand at 131.280 kb on + strand at 131.281 kb on - strand at 131.282 kb on + strand at 131.283 kb on - strand at 131.330 kb on + strand at 131.374 kb on - strand at 131.376 kb on - strand at 131.451 kb on - strand at 131.511 kb on + strand at 131.511 kb on + strand at 131.512 kb on - strand at 131.521 kb on + strand at 131.535 kb on + strand at 131.571 kb on - strand at 131.696 kb on + strand, within BPHYT_RS00545 at 131.696 kb on + strand, within BPHYT_RS00545 at 131.697 kb on - strand, within BPHYT_RS00545 at 131.697 kb on - strand, within BPHYT_RS00545 at 131.697 kb on - strand, within BPHYT_RS00545 at 131.777 kb on + strand, within BPHYT_RS00545 at 131.804 kb on - strand, within BPHYT_RS00545 at 131.804 kb on - strand, within BPHYT_RS00545 at 131.880 kb on - strand, within BPHYT_RS00545 at 131.949 kb on + strand, within BPHYT_RS00545 at 132.047 kb on + strand, within BPHYT_RS00545 at 132.047 kb on + strand, within BPHYT_RS00545
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 129,751 + BPHYT_RS00535 0.48 +0.1 129,752 - BPHYT_RS00535 0.48 -0.7 129,771 - BPHYT_RS00535 0.50 -0.5 129,771 - BPHYT_RS00535 0.50 -0.1 129,899 + BPHYT_RS00535 0.63 -0.2 129,900 - BPHYT_RS00535 0.63 +0.5 130,111 + BPHYT_RS00535 0.85 -0.5 130,111 + BPHYT_RS00535 0.85 -0.1 130,135 + BPHYT_RS00535 0.88 +1.2 130,136 - BPHYT_RS00535 0.88 -0.4 130,188 + -0.4 130,189 - -1.1 130,311 - -1.8 130,432 - -0.3 130,514 + +0.5 130,515 - -0.1 130,515 - -1.3 130,586 - -0.4 130,916 - BPHYT_RS00540 0.62 -1.8 130,951 + BPHYT_RS00540 0.70 +0.7 130,969 + BPHYT_RS00540 0.74 -1.1 130,979 - BPHYT_RS00540 0.77 -0.9 131,152 + -0.2 131,280 + -0.3 131,280 + -2.3 131,281 - +1.6 131,282 + -0.9 131,283 - +0.6 131,330 + -1.2 131,374 - +0.0 131,376 - -1.3 131,451 - -0.0 131,511 + +0.3 131,511 + -0.7 131,512 - -0.4 131,521 + -1.0 131,535 + +0.9 131,571 - +0.2 131,696 + BPHYT_RS00545 0.19 -1.1 131,696 + BPHYT_RS00545 0.19 -0.8 131,697 - BPHYT_RS00545 0.19 -0.3 131,697 - BPHYT_RS00545 0.19 +1.3 131,697 - BPHYT_RS00545 0.19 +0.4 131,777 + BPHYT_RS00545 0.28 +0.3 131,804 - BPHYT_RS00545 0.31 +0.6 131,804 - BPHYT_RS00545 0.31 -1.9 131,880 - BPHYT_RS00545 0.39 -0.5 131,949 + BPHYT_RS00545 0.46 +0.7 132,047 + BPHYT_RS00545 0.57 +0.4 132,047 + BPHYT_RS00545 0.57 +3.4
Or see this region's nucleotide sequence