Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28035

Experiment: 36hr PHYTO3 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28030 and BPHYT_RS28035 are separated by 72 nucleotidesBPHYT_RS28035 and BPHYT_RS28040 are separated by 111 nucleotides BPHYT_RS28030: BPHYT_RS28030 - methylmalonate-semialdehyde dehydrogenase, at 1,851,390 to 1,852,901 _RS28030 BPHYT_RS28035: BPHYT_RS28035 - AMP-dependent synthetase, at 1,852,974 to 1,854,665 _RS28035 BPHYT_RS28040: BPHYT_RS28040 - acyl-CoA dehydrogenase, at 1,854,777 to 1,855,910 _RS28040 Position (kb) 1852 1853 1854 1855Strain fitness (log2 ratio) -2 -1 0 1at 1852.483 kb on + strand, within BPHYT_RS28030at 1852.483 kb on + strand, within BPHYT_RS28030at 1852.484 kb on - strand, within BPHYT_RS28030at 1852.484 kb on - strand, within BPHYT_RS28030at 1853.098 kb on - strandat 1853.660 kb on + strand, within BPHYT_RS28035at 1854.457 kb on + strand, within BPHYT_RS28035at 1854.604 kb on + strandat 1854.625 kb on - strandat 1854.690 kb on - strandat 1854.778 kb on + strandat 1854.778 kb on + strandat 1854.778 kb on + strandat 1854.778 kb on + strandat 1855.310 kb on + strand, within BPHYT_RS28040at 1855.310 kb on + strand, within BPHYT_RS28040at 1855.310 kb on + strand, within BPHYT_RS28040at 1855.641 kb on - strand, within BPHYT_RS28040

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 0.1
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1,852,483 + BPHYT_RS28030 0.72 +0.9
1,852,483 + BPHYT_RS28030 0.72 +1.1
1,852,484 - BPHYT_RS28030 0.72 -1.9
1,852,484 - BPHYT_RS28030 0.72 -0.0
1,853,098 - +1.0
1,853,660 + BPHYT_RS28035 0.41 +1.7
1,854,457 + BPHYT_RS28035 0.88 +1.0
1,854,604 + +0.7
1,854,625 - +0.4
1,854,690 - -0.3
1,854,778 + +0.3
1,854,778 + +0.1
1,854,778 + +0.8
1,854,778 + +0.5
1,855,310 + BPHYT_RS28040 0.47 +0.9
1,855,310 + BPHYT_RS28040 0.47 +1.1
1,855,310 + BPHYT_RS28040 0.47 +0.9
1,855,641 - BPHYT_RS28040 0.76 -0.1

Or see this region's nucleotide sequence