Experiment: 36hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25680 and BPHYT_RS25685 are separated by 233 nucleotides BPHYT_RS25685 and BPHYT_RS25690 are separated by 14 nucleotides BPHYT_RS25690 and BPHYT_RS25695 are separated by 56 nucleotides BPHYT_RS25695 and BPHYT_RS25700 are separated by 127 nucleotides
BPHYT_RS25680: BPHYT_RS25680 - GntR family transcriptional regulator, at 1,335,175 to 1,335,804
_RS25680
BPHYT_RS25685: BPHYT_RS25685 - damage-inducible protein DinB, at 1,336,038 to 1,336,556
_RS25685
BPHYT_RS25690: BPHYT_RS25690 - GntR family transcriptional regulator, at 1,336,571 to 1,337,380
_RS25690
BPHYT_RS25695: BPHYT_RS25695 - enoyl-CoA hydratase, at 1,337,437 to 1,338,168
_RS25695
BPHYT_RS25700: BPHYT_RS25700 - MFS transporter, at 1,338,296 to 1,339,696
_RS25700
Position (kb)
1336
1337
1338 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1335.915 kb on + strand at 1335.916 kb on - strand at 1336.148 kb on - strand, within BPHYT_RS25685 at 1336.335 kb on + strand at 1336.403 kb on - strand, within BPHYT_RS25685 at 1336.403 kb on - strand, within BPHYT_RS25685 at 1336.403 kb on - strand, within BPHYT_RS25685 at 1336.406 kb on + strand, within BPHYT_RS25685 at 1337.192 kb on + strand, within BPHYT_RS25690 at 1337.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 0.1 remove 1,335,915 + +0.2 1,335,916 - +0.3 1,336,148 - BPHYT_RS25685 0.21 -1.4 1,336,335 + +1.7 1,336,403 - BPHYT_RS25685 0.70 -0.4 1,336,403 - BPHYT_RS25685 0.70 -2.2 1,336,403 - BPHYT_RS25685 0.70 -3.2 1,336,406 + BPHYT_RS25685 0.71 -0.4 1,337,192 + BPHYT_RS25690 0.77 -0.1 1,337,422 - -0.6
Or see this region's nucleotide sequence