Experiment: 36hr PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00785 and BPHYT_RS00790 overlap by 4 nucleotides BPHYT_RS00790 and BPHYT_RS00795 are separated by 189 nucleotides BPHYT_RS00795 and BPHYT_RS00800 are separated by 14 nucleotides
BPHYT_RS00785: BPHYT_RS00785 - conjugal transfer protein TraH, at 178,278 to 179,435
_RS00785
BPHYT_RS00790: BPHYT_RS00790 - conjugal transfer protein TraF, at 179,432 to 180,142
_RS00790
BPHYT_RS00795: BPHYT_RS00795 - conjugal transfer protein TraJ, at 180,332 to 181,090
_RS00795
BPHYT_RS00800: BPHYT_RS00800 - Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9), at 181,105 to 181,932
_RS00800
Position (kb) 179
180
181 Strain fitness (log2 ratio) -2
-1
0
1 at 178.521 kb on + strand, within BPHYT_RS00785 at 178.521 kb on + strand, within BPHYT_RS00785 at 178.544 kb on - strand, within BPHYT_RS00785 at 178.671 kb on + strand, within BPHYT_RS00785 at 178.723 kb on - strand, within BPHYT_RS00785 at 178.969 kb on - strand, within BPHYT_RS00785 at 179.257 kb on - strand, within BPHYT_RS00785 at 179.381 kb on + strand at 179.532 kb on - strand, within BPHYT_RS00790 at 179.702 kb on - strand, within BPHYT_RS00790 at 179.702 kb on - strand, within BPHYT_RS00790 at 180.258 kb on - strand at 180.518 kb on + strand at 180.519 kb on - strand, within BPHYT_RS00795 at 180.642 kb on - strand, within BPHYT_RS00795 at 180.663 kb on - strand, within BPHYT_RS00795 at 180.746 kb on + strand, within BPHYT_RS00795 at 180.746 kb on + strand, within BPHYT_RS00795 at 181.005 kb on - strand, within BPHYT_RS00795 at 181.142 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 0.1 remove 178,521 + BPHYT_RS00785 0.21 -0.2 178,521 + BPHYT_RS00785 0.21 -0.0 178,544 - BPHYT_RS00785 0.23 -0.1 178,671 + BPHYT_RS00785 0.34 -0.0 178,723 - BPHYT_RS00785 0.38 -0.0 178,969 - BPHYT_RS00785 0.60 +0.5 179,257 - BPHYT_RS00785 0.85 +0.4 179,381 + -0.3 179,532 - BPHYT_RS00790 0.14 -0.0 179,702 - BPHYT_RS00790 0.38 -1.2 179,702 - BPHYT_RS00790 0.38 -2.2 180,258 - -0.2 180,518 + +1.7 180,519 - BPHYT_RS00795 0.25 -0.1 180,642 - BPHYT_RS00795 0.41 -0.7 180,663 - BPHYT_RS00795 0.44 -0.7 180,746 + BPHYT_RS00795 0.55 -0.6 180,746 + BPHYT_RS00795 0.55 -0.5 181,005 - BPHYT_RS00795 0.89 -1.0 181,142 + -1.6
Or see this region's nucleotide sequence