Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34080

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34070 and BPHYT_RS34075 overlap by 4 nucleotidesBPHYT_RS34075 and BPHYT_RS34080 are separated by 113 nucleotidesBPHYT_RS34080 and BPHYT_RS34085 are separated by 156 nucleotides BPHYT_RS34070: BPHYT_RS34070 - histidine kinase, at 3,242,582 to 3,242,968 _RS34070 BPHYT_RS34075: BPHYT_RS34075 - chemotaxis protein CheY, at 3,242,965 to 3,243,606 _RS34075 BPHYT_RS34080: BPHYT_RS34080 - flagellar motor protein MotB, at 3,243,720 to 3,244,808 _RS34080 BPHYT_RS34085: BPHYT_RS34085 - polyamine ABC transporter permease, at 3,244,965 to 3,245,804 _RS34085 Position (kb) 3243 3244 3245Strain fitness (log2 ratio) -2 -1 0 1 2at 3242.810 kb on - strand, within BPHYT_RS34070at 3242.810 kb on - strand, within BPHYT_RS34070at 3243.055 kb on - strand, within BPHYT_RS34075at 3243.082 kb on - strand, within BPHYT_RS34075at 3243.451 kb on + strand, within BPHYT_RS34075at 3243.472 kb on + strand, within BPHYT_RS34075at 3243.565 kb on - strandat 3243.597 kb on + strandat 3243.627 kb on - strandat 3243.922 kb on + strand, within BPHYT_RS34080at 3244.358 kb on + strand, within BPHYT_RS34080at 3244.640 kb on - strand, within BPHYT_RS34080at 3244.786 kb on - strandat 3244.852 kb on + strandat 3244.902 kb on + strandat 3244.962 kb on + strandat 3244.963 kb on - strandat 3244.974 kb on - strandat 3245.019 kb on + strandat 3245.019 kb on + strandat 3245.079 kb on - strand, within BPHYT_RS34085at 3245.117 kb on + strand, within BPHYT_RS34085at 3245.375 kb on + strand, within BPHYT_RS34085at 3245.771 kb on + strandat 3245.772 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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3,242,810 - BPHYT_RS34070 0.59 +0.1
3,242,810 - BPHYT_RS34070 0.59 -0.2
3,243,055 - BPHYT_RS34075 0.14 +0.8
3,243,082 - BPHYT_RS34075 0.18 +0.5
3,243,451 + BPHYT_RS34075 0.76 -0.9
3,243,472 + BPHYT_RS34075 0.79 -0.2
3,243,565 - -0.9
3,243,597 + +0.7
3,243,627 - -0.1
3,243,922 + BPHYT_RS34080 0.19 +0.7
3,244,358 + BPHYT_RS34080 0.59 +1.5
3,244,640 - BPHYT_RS34080 0.84 +0.3
3,244,786 - -0.3
3,244,852 + +2.1
3,244,902 + +0.5
3,244,962 + +0.4
3,244,963 - -0.0
3,244,974 - -2.1
3,245,019 + -1.0
3,245,019 + -0.7
3,245,079 - BPHYT_RS34085 0.14 +1.8
3,245,117 + BPHYT_RS34085 0.18 +0.0
3,245,375 + BPHYT_RS34085 0.49 -0.9
3,245,771 + -0.3
3,245,772 - -0.4

Or see this region's nucleotide sequence