Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34035

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34030 and BPHYT_RS34035 are separated by 24 nucleotidesBPHYT_RS34035 and BPHYT_RS34040 are separated by 21 nucleotides BPHYT_RS34030: BPHYT_RS34030 - pilus assembly protein CpaB, at 3,234,070 to 3,235,170 _RS34030 BPHYT_RS34035: BPHYT_RS34035 - type II and III secretion system outermembrane protein, secretin, at 3,235,195 to 3,236,880 _RS34035 BPHYT_RS34040: BPHYT_RS34040 - hypothetical protein, at 3,236,902 to 3,238,806 _RS34040 Position (kb) 3235 3236 3237Strain fitness (log2 ratio) -1 0 1at 3234.567 kb on - strand, within BPHYT_RS34030at 3234.569 kb on - strand, within BPHYT_RS34030at 3234.740 kb on - strand, within BPHYT_RS34030at 3234.858 kb on + strand, within BPHYT_RS34030at 3235.099 kb on + strandat 3235.901 kb on - strand, within BPHYT_RS34035at 3236.157 kb on + strand, within BPHYT_RS34035at 3236.235 kb on + strand, within BPHYT_RS34035at 3236.999 kb on + strandat 3236.999 kb on + strandat 3237.000 kb on - strandat 3237.003 kb on + strandat 3237.061 kb on + strandat 3237.062 kb on - strandat 3237.411 kb on + strand, within BPHYT_RS34040at 3237.412 kb on - strand, within BPHYT_RS34040

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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3,234,567 - BPHYT_RS34030 0.45 +0.3
3,234,569 - BPHYT_RS34030 0.45 -1.0
3,234,740 - BPHYT_RS34030 0.61 -0.3
3,234,858 + BPHYT_RS34030 0.72 -1.3
3,235,099 + +0.6
3,235,901 - BPHYT_RS34035 0.42 +1.1
3,236,157 + BPHYT_RS34035 0.57 +1.5
3,236,235 + BPHYT_RS34035 0.62 +0.5
3,236,999 + +0.2
3,236,999 + -0.5
3,237,000 - +0.8
3,237,003 + -0.5
3,237,061 + +1.1
3,237,062 - +0.4
3,237,411 + BPHYT_RS34040 0.27 +0.8
3,237,412 - BPHYT_RS34040 0.27 +0.1

Or see this region's nucleotide sequence