Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33730 and BPHYT_RS33735 are separated by 112 nucleotides BPHYT_RS33735 and BPHYT_RS33740 overlap by 4 nucleotides BPHYT_RS33740 and BPHYT_RS33745 are separated by 103 nucleotides BPHYT_RS33745 and BPHYT_RS33750 are separated by 144 nucleotides
BPHYT_RS33730: BPHYT_RS33730 - hypothetical protein, at 3,150,861 to 3,151,433
_RS33730
BPHYT_RS33735: BPHYT_RS33735 - hypothetical protein, at 3,151,546 to 3,151,983
_RS33735
BPHYT_RS33740: BPHYT_RS33740 - hypothetical protein, at 3,151,980 to 3,152,681
_RS33740
BPHYT_RS33745: BPHYT_RS33745 - metallophosphoesterase, at 3,152,785 to 3,153,522
_RS33745
BPHYT_RS33750: BPHYT_RS33750 - cytochrome C, at 3,153,667 to 3,155,091
_RS33750
Position (kb)
3151
3152
3153 Strain fitness (log2 ratio)
-1
0
1 at 3151.477 kb on - strand at 3151.477 kb on - strand at 3151.478 kb on + strand at 3151.478 kb on + strand at 3151.479 kb on - strand at 3151.479 kb on - strand at 3151.723 kb on + strand, within BPHYT_RS33735 at 3151.804 kb on + strand, within BPHYT_RS33735 at 3151.804 kb on + strand, within BPHYT_RS33735 at 3151.804 kb on + strand, within BPHYT_RS33735 at 3151.804 kb on + strand, within BPHYT_RS33735 at 3151.805 kb on - strand, within BPHYT_RS33735 at 3152.116 kb on + strand, within BPHYT_RS33740 at 3152.116 kb on + strand, within BPHYT_RS33740 at 3152.118 kb on + strand, within BPHYT_RS33740 at 3152.475 kb on + strand, within BPHYT_RS33740 at 3152.626 kb on - strand at 3152.626 kb on - strand at 3152.708 kb on - strand at 3152.874 kb on + strand, within BPHYT_RS33745 at 3152.875 kb on - strand, within BPHYT_RS33745 at 3153.227 kb on + strand, within BPHYT_RS33745 at 3153.533 kb on + strand at 3153.662 kb on - strand at 3153.668 kb on + strand at 3153.669 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 3,151,477 - -1.5 3,151,477 - -0.4 3,151,478 + -0.9 3,151,478 + +0.1 3,151,479 - +0.7 3,151,479 - -0.1 3,151,723 + BPHYT_RS33735 0.40 -0.7 3,151,804 + BPHYT_RS33735 0.59 -0.2 3,151,804 + BPHYT_RS33735 0.59 -0.3 3,151,804 + BPHYT_RS33735 0.59 +0.8 3,151,804 + BPHYT_RS33735 0.59 +1.1 3,151,805 - BPHYT_RS33735 0.59 -1.6 3,152,116 + BPHYT_RS33740 0.19 -1.3 3,152,116 + BPHYT_RS33740 0.19 -0.8 3,152,118 + BPHYT_RS33740 0.20 +0.5 3,152,475 + BPHYT_RS33740 0.71 -1.7 3,152,626 - -0.3 3,152,626 - -0.2 3,152,708 - -0.3 3,152,874 + BPHYT_RS33745 0.12 +0.3 3,152,875 - BPHYT_RS33745 0.12 -0.7 3,153,227 + BPHYT_RS33745 0.60 -0.8 3,153,533 + +0.7 3,153,662 - +0.1 3,153,668 + +1.1 3,153,669 - -0.3
Or see this region's nucleotide sequence