Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32730 and BPHYT_RS32735 are separated by 18 nucleotides BPHYT_RS32735 and BPHYT_RS32740 are separated by 109 nucleotides BPHYT_RS32740 and BPHYT_RS32745 are separated by 19 nucleotides
BPHYT_RS32730: BPHYT_RS32730 - histidine phosphatase, at 2,922,196 to 2,922,792
_RS32730
BPHYT_RS32735: BPHYT_RS32735 - hypothetical protein, at 2,922,811 to 2,923,251
_RS32735
BPHYT_RS32740: BPHYT_RS32740 - hypothetical protein, at 2,923,361 to 2,924,377
_RS32740
BPHYT_RS32745: BPHYT_RS32745 - major facilitator transporter, at 2,924,397 to 2,925,755
_RS32745
Position (kb)
2923
2924
2925 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2922.612 kb on + strand, within BPHYT_RS32730 at 2922.705 kb on - strand, within BPHYT_RS32730 at 2922.867 kb on + strand, within BPHYT_RS32735 at 2922.896 kb on + strand, within BPHYT_RS32735 at 2922.897 kb on - strand, within BPHYT_RS32735 at 2923.028 kb on + strand, within BPHYT_RS32735 at 2923.285 kb on + strand at 2923.285 kb on + strand at 2923.492 kb on + strand, within BPHYT_RS32740 at 2923.494 kb on + strand, within BPHYT_RS32740 at 2923.669 kb on - strand, within BPHYT_RS32740 at 2923.692 kb on + strand, within BPHYT_RS32740 at 2923.791 kb on + strand, within BPHYT_RS32740 at 2923.791 kb on + strand, within BPHYT_RS32740 at 2923.792 kb on - strand, within BPHYT_RS32740 at 2923.792 kb on - strand, within BPHYT_RS32740 at 2923.872 kb on + strand, within BPHYT_RS32740 at 2924.172 kb on + strand, within BPHYT_RS32740 at 2924.173 kb on - strand, within BPHYT_RS32740 at 2924.173 kb on - strand, within BPHYT_RS32740 at 2924.383 kb on - strand at 2924.456 kb on - strand at 2924.750 kb on - strand, within BPHYT_RS32745 at 2924.782 kb on + strand, within BPHYT_RS32745 at 2924.914 kb on + strand, within BPHYT_RS32745 at 2924.926 kb on + strand, within BPHYT_RS32745 at 2924.926 kb on + strand, within BPHYT_RS32745 at 2924.927 kb on - strand, within BPHYT_RS32745 at 2924.927 kb on - strand, within BPHYT_RS32745 at 2924.942 kb on - strand, within BPHYT_RS32745 at 2925.098 kb on - strand, within BPHYT_RS32745
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,922,612 + BPHYT_RS32730 0.70 +1.3 2,922,705 - BPHYT_RS32730 0.85 +0.6 2,922,867 + BPHYT_RS32735 0.13 +1.0 2,922,896 + BPHYT_RS32735 0.19 +0.1 2,922,897 - BPHYT_RS32735 0.20 +0.8 2,923,028 + BPHYT_RS32735 0.49 +0.0 2,923,285 + -0.1 2,923,285 + -1.0 2,923,492 + BPHYT_RS32740 0.13 -0.4 2,923,494 + BPHYT_RS32740 0.13 -1.0 2,923,669 - BPHYT_RS32740 0.30 -0.4 2,923,692 + BPHYT_RS32740 0.33 +2.7 2,923,791 + BPHYT_RS32740 0.42 +0.3 2,923,791 + BPHYT_RS32740 0.42 -0.5 2,923,792 - BPHYT_RS32740 0.42 -0.2 2,923,792 - BPHYT_RS32740 0.42 -1.0 2,923,872 + BPHYT_RS32740 0.50 +0.5 2,924,172 + BPHYT_RS32740 0.80 +1.4 2,924,173 - BPHYT_RS32740 0.80 +0.5 2,924,173 - BPHYT_RS32740 0.80 -1.8 2,924,383 - -1.7 2,924,456 - +0.7 2,924,750 - BPHYT_RS32745 0.26 -0.2 2,924,782 + BPHYT_RS32745 0.28 -0.6 2,924,914 + BPHYT_RS32745 0.38 +0.8 2,924,926 + BPHYT_RS32745 0.39 +0.4 2,924,926 + BPHYT_RS32745 0.39 +1.4 2,924,927 - BPHYT_RS32745 0.39 -0.7 2,924,927 - BPHYT_RS32745 0.39 -0.8 2,924,942 - BPHYT_RS32745 0.40 +0.7 2,925,098 - BPHYT_RS32745 0.52 -0.6
Or see this region's nucleotide sequence