Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31255

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31245 and BPHYT_RS31250 overlap by 4 nucleotidesBPHYT_RS31250 and BPHYT_RS31255 are separated by 150 nucleotidesBPHYT_RS31255 and BPHYT_RS31260 are separated by 206 nucleotides BPHYT_RS31245: BPHYT_RS31245 - peptide ABC transporter ATP-binding protein, at 2,568,848 to 2,569,726 _RS31245 BPHYT_RS31250: BPHYT_RS31250 - ABC transporter, at 2,569,723 to 2,570,505 _RS31250 BPHYT_RS31255: BPHYT_RS31255 - cytochrome B561, at 2,570,656 to 2,571,231 _RS31255 BPHYT_RS31260: BPHYT_RS31260 - histidine kinase, at 2,571,438 to 2,572,127 _RS31260 Position (kb) 2570 2571 2572Strain fitness (log2 ratio) -1 0 1at 2569.722 kb on + strandat 2570.125 kb on + strand, within BPHYT_RS31250at 2570.943 kb on - strand, within BPHYT_RS31255at 2571.025 kb on + strandat 2571.187 kb on + strandat 2571.334 kb on - strandat 2571.850 kb on - strand, within BPHYT_RS31260at 2572.130 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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2,569,722 + +0.5
2,570,125 + BPHYT_RS31250 0.51 -1.0
2,570,943 - BPHYT_RS31255 0.50 -1.1
2,571,025 + +1.7
2,571,187 + +0.8
2,571,334 - -1.7
2,571,850 - BPHYT_RS31260 0.60 +0.6
2,572,130 + -0.4

Or see this region's nucleotide sequence