Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30330 and BPHYT_RS30335 are separated by 11 nucleotides BPHYT_RS30335 and BPHYT_RS30340 are separated by 25 nucleotides BPHYT_RS30340 and BPHYT_RS30345 are separated by 20 nucleotides BPHYT_RS30345 and BPHYT_RS30350 are separated by 222 nucleotides
BPHYT_RS30330: BPHYT_RS30330 - hypothetical protein, at 2,360,135 to 2,360,803
_RS30330
BPHYT_RS30335: BPHYT_RS30335 - hypothetical protein, at 2,360,815 to 2,361,213
_RS30335
BPHYT_RS30340: BPHYT_RS30340 - AraC family transcriptional regulator, at 2,361,239 to 2,362,093
_RS30340
BPHYT_RS30345: BPHYT_RS30345 - alanyl-tRNA synthetase, at 2,362,114 to 2,362,827
_RS30345
BPHYT_RS30350: BPHYT_RS30350 - LysR family transcriptional regulator, at 2,363,050 to 2,363,970
_RS30350
Position (kb)
2361
2362
2363 Strain fitness (log2 ratio)
-1
0
1
2 at 2360.558 kb on + strand, within BPHYT_RS30330 at 2360.611 kb on - strand, within BPHYT_RS30330 at 2361.031 kb on + strand, within BPHYT_RS30335 at 2361.032 kb on - strand, within BPHYT_RS30335 at 2361.460 kb on - strand, within BPHYT_RS30340 at 2361.817 kb on - strand, within BPHYT_RS30340 at 2362.079 kb on - strand at 2362.347 kb on - strand, within BPHYT_RS30345 at 2362.506 kb on - strand, within BPHYT_RS30345 at 2362.536 kb on + strand, within BPHYT_RS30345 at 2362.857 kb on - strand at 2362.984 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,360,558 + BPHYT_RS30330 0.63 +2.1 2,360,611 - BPHYT_RS30330 0.71 +0.5 2,361,031 + BPHYT_RS30335 0.54 -0.6 2,361,032 - BPHYT_RS30335 0.54 -0.1 2,361,460 - BPHYT_RS30340 0.26 +1.5 2,361,817 - BPHYT_RS30340 0.68 -1.6 2,362,079 - +0.1 2,362,347 - BPHYT_RS30345 0.33 -1.2 2,362,506 - BPHYT_RS30345 0.55 +0.2 2,362,536 + BPHYT_RS30345 0.59 -1.4 2,362,857 - -1.1 2,362,984 - +0.5
Or see this region's nucleotide sequence