Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30305 and BPHYT_RS30310 are separated by 29 nucleotides BPHYT_RS30310 and BPHYT_RS30315 overlap by 4 nucleotides BPHYT_RS30315 and BPHYT_RS30320 are separated by 127 nucleotides
BPHYT_RS30305: BPHYT_RS30305 - ethyl tert-butyl ether degradation EthD, at 2,354,953 to 2,355,663
_RS30305
BPHYT_RS30310: BPHYT_RS30310 - LysR family transcriptional regulator, at 2,355,693 to 2,356,643
_RS30310
BPHYT_RS30315: BPHYT_RS30315 - LysR family transcriptional regulator, at 2,356,640 to 2,357,539
_RS30315
BPHYT_RS30320: BPHYT_RS30320 - MFS transporter, at 2,357,667 to 2,358,989
_RS30320
Position (kb)
2356
2357
2358 Strain fitness (log2 ratio)
-1
0
1 at 2355.645 kb on + strand at 2356.585 kb on - strand at 2356.946 kb on + strand, within BPHYT_RS30315 at 2357.114 kb on - strand, within BPHYT_RS30315 at 2357.114 kb on - strand, within BPHYT_RS30315 at 2357.559 kb on + strand at 2357.751 kb on + strand at 2357.885 kb on - strand, within BPHYT_RS30320 at 2358.049 kb on - strand, within BPHYT_RS30320 at 2358.100 kb on - strand, within BPHYT_RS30320 at 2358.163 kb on - strand, within BPHYT_RS30320 at 2358.438 kb on - strand, within BPHYT_RS30320
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,355,645 + -0.2 2,356,585 - -1.0 2,356,946 + BPHYT_RS30315 0.34 -1.3 2,357,114 - BPHYT_RS30315 0.53 -1.2 2,357,114 - BPHYT_RS30315 0.53 +0.0 2,357,559 + +1.1 2,357,751 + -0.4 2,357,885 - BPHYT_RS30320 0.16 +0.0 2,358,049 - BPHYT_RS30320 0.29 +0.1 2,358,100 - BPHYT_RS30320 0.33 -0.1 2,358,163 - BPHYT_RS30320 0.37 -0.7 2,358,438 - BPHYT_RS30320 0.58 -0.5
Or see this region's nucleotide sequence