Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27945

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27940 and BPHYT_RS27945 are separated by 302 nucleotidesBPHYT_RS27945 and BPHYT_RS27950 are separated by 14 nucleotidesBPHYT_RS27950 and BPHYT_RS27955 overlap by 4 nucleotides BPHYT_RS27940: BPHYT_RS27940 - FAD-binding protein, at 1,834,438 to 1,835,289 _RS27940 BPHYT_RS27945: BPHYT_RS27945 - dihydroxyacetone kinase, at 1,835,592 to 1,836,581 _RS27945 BPHYT_RS27950: BPHYT_RS27950 - dihydroxyacetone kinase, at 1,836,596 to 1,837,228 _RS27950 BPHYT_RS27955: BPHYT_RS27955 - ABC transporter ATP-binding protein, at 1,837,225 to 1,838,262 _RS27955 Position (kb) 1835 1836 1837Strain fitness (log2 ratio) -1 0 1at 1834.890 kb on + strand, within BPHYT_RS27940at 1835.054 kb on + strand, within BPHYT_RS27940at 1835.099 kb on + strand, within BPHYT_RS27940at 1835.119 kb on + strand, within BPHYT_RS27940at 1835.143 kb on + strand, within BPHYT_RS27940at 1835.783 kb on - strand, within BPHYT_RS27945at 1836.494 kb on + strandat 1836.670 kb on + strand, within BPHYT_RS27950at 1836.670 kb on + strand, within BPHYT_RS27950at 1836.851 kb on - strand, within BPHYT_RS27950at 1836.851 kb on - strand, within BPHYT_RS27950at 1837.169 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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1,834,890 + BPHYT_RS27940 0.53 +0.8
1,835,054 + BPHYT_RS27940 0.72 +0.6
1,835,099 + BPHYT_RS27940 0.78 +0.1
1,835,119 + BPHYT_RS27940 0.80 +0.3
1,835,143 + BPHYT_RS27940 0.83 +0.7
1,835,783 - BPHYT_RS27945 0.19 -0.0
1,836,494 + +0.8
1,836,670 + BPHYT_RS27950 0.12 -0.1
1,836,670 + BPHYT_RS27950 0.12 +0.2
1,836,851 - BPHYT_RS27950 0.40 -1.5
1,836,851 - BPHYT_RS27950 0.40 +0.5
1,837,169 - +0.5

Or see this region's nucleotide sequence