Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27735

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27725 and BPHYT_RS27730 are separated by 46 nucleotidesBPHYT_RS27730 and BPHYT_RS27735 are separated by 171 nucleotidesBPHYT_RS27735 and BPHYT_RS27740 are separated by 37 nucleotidesBPHYT_RS27740 and BPHYT_RS27745 are separated by 297 nucleotides BPHYT_RS27725: BPHYT_RS27725 - hypothetical protein, at 1,783,832 to 1,784,050 _RS27725 BPHYT_RS27730: BPHYT_RS27730 - LuxR family transcriptional regulator, at 1,784,097 to 1,784,744 _RS27730 BPHYT_RS27735: BPHYT_RS27735 - nitroreductase, at 1,784,916 to 1,785,947 _RS27735 BPHYT_RS27740: BPHYT_RS27740 - hypothetical protein, at 1,785,985 to 1,786,260 _RS27740 BPHYT_RS27745: BPHYT_RS27745 - nitroreductase, at 1,786,558 to 1,787,229 _RS27745 Position (kb) 1784 1785 1786Strain fitness (log2 ratio) -2 -1 0 1 2at 1783.943 kb on - strand, within BPHYT_RS27725at 1784.799 kb on + strandat 1784.888 kb on + strandat 1785.204 kb on + strand, within BPHYT_RS27735at 1785.205 kb on - strand, within BPHYT_RS27735at 1785.616 kb on + strand, within BPHYT_RS27735at 1785.663 kb on - strand, within BPHYT_RS27735at 1785.663 kb on - strand, within BPHYT_RS27735at 1785.937 kb on + strandat 1785.938 kb on - strandat 1786.520 kb on + strandat 1786.520 kb on + strandat 1786.647 kb on - strand, within BPHYT_RS27745at 1786.844 kb on - strand, within BPHYT_RS27745

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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1,783,943 - BPHYT_RS27725 0.51 +0.6
1,784,799 + +0.2
1,784,888 + -1.9
1,785,204 + BPHYT_RS27735 0.28 +2.1
1,785,205 - BPHYT_RS27735 0.28 -2.1
1,785,616 + BPHYT_RS27735 0.68 -1.4
1,785,663 - BPHYT_RS27735 0.72 +1.7
1,785,663 - BPHYT_RS27735 0.72 +0.6
1,785,937 + +0.1
1,785,938 - -0.1
1,786,520 + -1.0
1,786,520 + -0.1
1,786,647 - BPHYT_RS27745 0.13 +0.4
1,786,844 - BPHYT_RS27745 0.43 -0.3

Or see this region's nucleotide sequence