Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27710 and BPHYT_RS27715 are separated by 173 nucleotides BPHYT_RS27715 and BPHYT_RS27720 overlap by 2 nucleotides BPHYT_RS27720 and BPHYT_RS27725 are separated by 0 nucleotides BPHYT_RS27725 and BPHYT_RS27730 are separated by 46 nucleotides BPHYT_RS27730 and BPHYT_RS27735 are separated by 171 nucleotides
BPHYT_RS27710: BPHYT_RS27710 - universal stress protein A, at 1,782,113 to 1,782,943
_RS27710
BPHYT_RS27715: BPHYT_RS27715 - UspA domain-containing protein, at 1,783,117 to 1,783,593
_RS27715
BPHYT_RS27720: BPHYT_RS27720 - hypothetical protein, at 1,783,592 to 1,783,831
_RS27720
BPHYT_RS27725: BPHYT_RS27725 - hypothetical protein, at 1,783,832 to 1,784,050
_RS27725
BPHYT_RS27730: BPHYT_RS27730 - LuxR family transcriptional regulator, at 1,784,097 to 1,784,744
_RS27730
BPHYT_RS27735: BPHYT_RS27735 - nitroreductase, at 1,784,916 to 1,785,947
_RS27735
Position (kb) 1783
1784
1785 Strain fitness (log2 ratio) -2
-1
0
1 at 1783.014 kb on - strand at 1783.417 kb on - strand, within BPHYT_RS27715 at 1783.679 kb on - strand, within BPHYT_RS27720 at 1783.943 kb on - strand, within BPHYT_RS27725 at 1784.799 kb on + strand at 1784.888 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,783,014 - -1.3 1,783,417 - BPHYT_RS27715 0.63 -0.6 1,783,679 - BPHYT_RS27720 0.36 -1.1 1,783,943 - BPHYT_RS27725 0.51 +0.6 1,784,799 + +0.2 1,784,888 + -1.9
Or see this region's nucleotide sequence